Isolating plasma extracellular vesicles from mouse blood using size-exclusion chromatography, density gradient, and ultracentrifugation

被引：6
|
作者：
Andre-Gregoire, Gwennan [1 ,2 ,3 ]
Roux, Quentin [1 ,2 ]
Gavard, Julie [1 ,2 ,3 ]
机构：
[1] Univ Angers, Nantes Univ, Team SOAP, INSERM,CRCI2NA,CNRS, F-44000 Nantes, France
[2] Equipe Labellisee Ligue Natl Canc, Aramis Project Team, F-75013 Paris, France
[3] Inst Cancerol Ouest ICO, Res Pathol Platform, F-44800 St herblain, France
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
关键词：
Cancer; Cell separation/fractionation; Molecular Biology;
D O I：
10.1016/j.xpro.2023.102740
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Circulating extracellular vesicles (EVs) could serve for the surveillance of diverse pathological conditions. We present a protocol for enriching and isolating plasma EVs from mouse blood. We describe steps for employing ultracentrifuga-tion, size-exclusion chromatography, and density gradients, required for further quantitative and qualitative analysis. We detail the procedure for retrieving optimal volume of blood while preserving its integrity and avoiding hemolysis. We also describe the preparation of EVs from this complex fluid containing soluble proteins, aggregates, and lipoprotein particles.For complete details on the use and execution of this protocol, please refer to Andre ' -Gre ' goire et al. (2022).1
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收藏
页数：19
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