MiR-155 enhances phagocytosis of alveolar macrophages through the mTORC2/RhoA pathway

被引:2
|
作者
Yang, Xinna [1 ,2 ]
Zeng, Xiaoli [1 ,2 ]
Shu, Juan [1 ,2 ]
Bao, Hairong [1 ,2 ]
Liu, Xiaoju [1 ,2 ]
机构
[1] Lanzhou Univ, Sch Clin Med 1, Lanzhou, Peoples R China
[2] Lanzhou Univ, Dept Gerontal Resp Med, Hosp 1, Lanzhou 730000, Peoples R China
基金
中国国家自然科学基金;
关键词
alveolar macrophage; cytoskeleton; MICRORNA-155; mTORC2; phagocytosis; MTOR; EXPRESSION; APOPTOSIS; MOTILITY; GROWTH; RICTOR;
D O I
10.1097/MD.0000000000034592
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Alveolar macrophage phagocytosis is significantly reduced in Chronic obstructive pulmonary disease, and cigarette smoke extract is one of the chief reasons for this decrease. Nevertheless, the specific underlying mechanism remains elusive. In this study, the role and possible mechanism of miR-155-5p/mTORC2/RhoA in the phagocytosis of mouse alveolar macrophages (MH-S) were explored. Our results revealed that cigarette smoke extract intervention reduced MH-S cell phagocytosis and miR-155-5p expression. Meanwhile, the dual-luciferase reporter assay validated that Rictor is a target of miR-155-5p. On the one hand, transfecting miR-155-5p mimic, mimic NC, miR-155-5p inhibitor, or inhibitor NC in MH-S cells overexpressing miR-155-5p increased the Alveolar macrophage phagocytotic rate, up-regulated the expression level of RhoA and p-RhoA, and down-regulated that of mTOR and Rictor mRNA and protein. On the other hand, inhibiting the expression of miR-155-5p lowered the phagocytotic rate, up-regulated the expression of mTOR, Rictor mRNA, and protein, and down-regulated the expression of RhoA and p-RhoA, which taken together, authenticated that miR-155-5p participates in macrophage phagocytosis via the mTORC2/RhoA pathway. Finally, confocal microscopy demonstrated that cells overexpressing miR-155-5p underwent cytoskeletal rearrangement during phagocytosis, and the phagocytic function of cells was enhanced, signaling that miR-155-5p participated in macrophage skeletal rearrangement and enhanced alveolar macrophage phagocytosis by targeting the expression of Rictor in the mTORC2/RhoA pathway.
引用
收藏
页数:8
相关论文
共 50 条
  • [41] Improving Type 2 Diabetes Through a Distinct Adrenergic Signaling Pathway Involving mTORC2 That Mediates Glucose Uptake in Skeletal Muscle
    Sato, Masaaki
    Dehvari, Nodi
    Oeberg, Anette I.
    Dallner, Olof S.
    Sandstrom, Anna L.
    Olsen, Jessica M.
    Csikasz, Robert I.
    Summers, Roger J.
    Hutchinson, Dana S.
    Bengtsson, Tore
    DIABETES, 2014, 63 (12) : 4115 - 4129
  • [42] The mTORC2 complex regulates C2C12 myoblast fusion through a ROCK-1-dependent pathway
    Shu, Lili
    Zhan, Jun
    Houghton, Peter
    CANCER RESEARCH, 2009, 69
  • [43] PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway
    Lv, Nianyin
    Jin, Sufeng
    Liang, Zihao
    Wu, Xiaohui
    Kang, Yanhua
    Su, Lan
    Dong, Yeping
    Wang, Bingwei
    Ma, Tonghui
    Shi, Liyun
    FRONTIERS IN IMMUNOLOGY, 2022, 12
  • [44] MiR-155 promotes proliferation and inhibits apoptosis of nasopharyngeal carcinoma cells through targeting PTEN-PI3K/AKT pathway
    Zuo, W-N
    Zhu, H.
    Li, L-P
    Jin, A-Y
    Wang, H-Q
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2019, 23 (18) : 7935 - 7942
  • [45] MiR-451a enhances the phagocytosis and affects both M1 and M2 polarization in macrophages
    Liu, Xiaoli
    Zhang, Dongyue
    Wang, Hao
    Ren, Qian
    Wang, Lina
    Zheng, Guoguang
    CELLULAR IMMUNOLOGY, 2021, 365
  • [46] DI-(2-ETHYLHEXYL) PHTHALATE ENHANCES THE RELEASE OF LYSOSOMAL-ENZYMES FROM ALVEOLAR MACROPHAGES DURING PHAGOCYTOSIS
    BALLY, MB
    OPHEIM, DJ
    SHERTZER, HG
    TOXICOLOGY, 1980, 18 (01) : 49 - 60
  • [47] PINK1/BRPK inhibits apoptotic cell death and enahances cellular invasiveness through an activation of mTORC2 pathway
    Hitoshi Murata
    Masakiyo Sakaguchi
    Ken Kataoka
    Nam-ho Huh
    BMC Proceedings, 4 (Suppl 2)
  • [48] Targeting CD47/TNFAIP8 by miR-155 overcomes drug resistance and inhibits tumor growth through induction of phagocytosis and apoptosis in multiple myeloma
    Rastgoo, Nasrin
    Wu, Jian
    Liu, Aijun
    Pourabdollah, Maryam
    Atenafu, Eshetu G.
    Reece, Donna
    Chen, Wenming
    Chang, Hong
    HAEMATOLOGICA, 2020, 105 (12) : 2813 - 2823
  • [49] The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through a Toll-like receptor 2-mediated signalling pathway
    Mae, Masako
    Iyori, Mitsuhiro
    Yasuda, Motoaki
    Shamsul, Haque Mohammad
    Kataoka, Hideo
    Kiura, Kazuto
    Hasebe, Akira
    Totsuka, Yasunori
    Shibata, Ken-ichiro
    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2007, 49 (03): : 398 - 409
  • [50] Targeting CD47 and TNFAIP8 By Mir-155 Overcomes Drug Resistance and Inhibits Tumor Growth through Induction of Phagocytosis and Apoptosis in Multiple Myeloma
    Rastgoo, Nasrin
    Abdi, Jahangir
    Pourabdollah, Maryam
    Chang, Hong
    BLOOD, 2018, 132