Simultaneous Analysis of Cyanotoxins β-N-methylamino-L-alanine (BMAA) and Microcystins-RR, -LR, and -YR Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

被引:5
|
作者
Pravadali-Cekic, Sercan [1 ]
Vojvodic, Aleksandar [1 ]
Violi, Jake P. [2 ]
Mitrovic, Simon M. [3 ]
Rodgers, Kenneth J. [3 ]
Bishop, David P. [1 ]
机构
[1] Univ Technol Sydney, Hyphenated Mass Spectrometry Lab HyMAS, Sydney, NSW 2007, Australia
[2] Univ New South Wales, Sch Chem, Sydney, NSW 2033, Australia
[3] Univ Technol Sydney, Sch Life Sci, Sydney, NSW 2007, Australia
来源
MOLECULES | 2023年 / 28卷 / 18期
关键词
BMAA; microcystins; cyanotoxins; cyanobacteria; method validation; FRESH-WATER CYANOBACTERIA; NEUROTOXIN BMAA; NATURAL-WATER; AMINO-ACIDS; TOXINS; ISOMERS; SAMPLES; HEPATOTOXINS; NODULARIN; SHELLFISH;
D O I
10.3390/molecules28186733
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
beta-N-methylamino-L-alanine (BMAA) and its isomers, 2,4-diaminobutyric acid (2,4-DAB) and N-(2-aminoethyl)-glycine (AEG), along with microcystins (MCs)-RR, -LR, and -YR (the major MC congeners), are cyanotoxins that can cause detrimental health and environmental impacts during toxic blooms. Currently, there are no reverse-phase (RP) LC-MS/MS methods for the simultaneous detection and quantification of BMAA, its isomers, and the major MCs in a single analysis; therefore, multiple analyses are required to assess the toxic load of a sample. Here, we present a newly developed and validated method for the detection and quantification of BMAA, 2,4-DAB, AEG, MC-LR, MC-RR, and MC-YR using RP LC-MS/MS. Method validation was performed, assessing linearity (r(2) > 0.996), accuracy (>90% recovery for spiked samples), precision (7% relative standard deviation), and limits of detection (LODs) and quantification (LOQs) (ranging from 0.13 to 1.38 ng mL(-1)). The application of this combined cyanotoxin analysis on a culture of Microcystis aeruginosa resulted in the simultaneous detection of 2,4-DAB (0.249 ng mg(-1) dry weight (DW)) and MC-YR (4828 ng mg(-1) DW). This study provides a unified method for the quantitative analysis of BMAA, its isomers, and three MC congeners in natural environmental samples.
引用
收藏
页数:17
相关论文
共 50 条
  • [11] Simultaneous Determination of 54 Pesticides in Proso Millet Using QuEChERS with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
    Ding, Chao
    Ren, Pengcheng
    Qi, Yanli
    Yang, Yanmei
    Qin, Shu
    MOLECULES, 2023, 28 (18):
  • [12] ESTIMATION OF MELOXICAM IN HUMAN PLASMA BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)
    Ganesh, Mani
    Hemalatha, Pushparaj
    Peng, Mei Mei
    Sakthimanigandan, Kalaimani
    Jang, Hyun Tae
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2013, 36 (07) : 867 - 880
  • [13] Quantification of psilocin in human whole blood using liquid chromatography-tandem mass spectrometry (LC-MS/MS)
    Gomonit, Munchelou M.
    Skillman, Britni
    Swortwood, Madeleine J.
    JOURNAL OF FORENSIC SCIENCES, 2024, 69 (02) : 678 - 687
  • [14] Measurement of Plasma Free Metanephrines Using a Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method
    MacMahon, M. M.
    Mulready, K. J.
    Fitzgibbon, M. C.
    IRISH JOURNAL OF MEDICAL SCIENCE, 2013, 182 : S406 - S406
  • [15] Analysis of methoxyfenozide residues in fruits, vegetables, and mint by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
    Hall, GL
    Engebretson, J
    Hengel, MJ
    Shibamoto, T
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) : 672 - 676
  • [16] Assessing Environmental Exposure to β-N-Methylamino-L-Alanine (BMAA) in Complex Sample Matrices: a Comparison of the Three Most Popular LC-MS/MS Methods
    Baker, Teesha C.
    Tymm, Fiona J. M.
    Murch, Susan J.
    NEUROTOXICITY RESEARCH, 2018, 33 (01) : 43 - 54
  • [17] Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
    Jensen, Berit Packert
    Vella-Brincat, Jane Winifred Ann
    Begg, Evan James
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (9-10): : 605 - 609
  • [18] Assessing Environmental Exposure to β-N-Methylamino-l-Alanine (BMAA) in Complex Sample Matrices: a Comparison of the Three Most Popular LC-MS/MS Methods
    Teesha C. Baker
    Fiona J. M. Tymm
    Susan J. Murch
    Neurotoxicity Research, 2018, 33 : 43 - 54
  • [19] Detection of cannabinoids in dried blood spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
    Paul, Heather
    Awad, Hanan
    Sadrzadeh, S. M. Hossein
    Boyd, Jessica
    CLINICAL BIOCHEMISTRY, 2019, 74 : 93 - 93
  • [20] Sensitive determination of paroxetine in canine plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
    Chang, Kyu Young
    Kang, Seung Woo
    Han, Sang Beom
    Youm, Jeong-Rok
    Lee, Kyung Ryul
    Lee, Hee Joo
    ANALYTICAL SCIENCE AND TECHNOLOGY, 2007, 20 (02): : 138 - 146