RUP2 facilitates UVR8 redimerization via two interfaces

被引：10

作者：

Wang, Lixia ^{[1
]}

Wang, Yidong ^{[1
]}

Chang, Hongfei ^{[1
]}

Ren, Hui ^{[2
]}

Wu, Xinquan ^{[2
]}

Wen, Jia ^{[2
]}

Guan, Zeyuan ^{[1
]}

Ma, Ling ^{[1
]}

Qiu, Liang ^{[1
]}

Yan, Junjie ^{[1
]}

Zhang, Delin ^{[1
]}

Huang, Xi ^{[2
]}

Yin, Ping ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

[2] Xiamen Univ, Fac Med & Life Sci, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China

来源：

PLANT COMMUNICATIONS | 2023年 / 4卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划; 中国博士后科学基金;

关键词：

photomorphogenesis; UV-B photoreceptor; UVR8; RUP2; COP1; B-INDUCED PHOTOMORPHOGENESIS; ULTRAVIOLET-B; PHOTORECEPTOR UVR8; SIGNAL-TRANSDUCTION; STRESS ACCLIMATION; GENE-EXPRESSION; TRANSCRIPTION; PERCEPTION; COP1; MECHANISM;

D O I：

10.1016/j.xplc.2022.100428

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) exists as a homodimer in its inactive ground state. Upon UV-B exposure, UVR8 monomerizes and interacts with a downstream key regulator, the CONSTITUTIVE PHOTOMORPHOGENIC 1/SUPPRESSOR OF PHYA (COP1/SPA) E3 ubiquitin ligase complex, to initiate UV-B signaling. Two WD40 proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2 directly interact with monomeric UVR8 and facilitate UVR8 ground state reversion, completing the UVR8 photocycle. Here, we reconstituted the RUP-mediated UVR8 redimerization process in vitro and reported the structure of the RUP2-UVR8(W285A) complex (2.0 angstrom). RUP2 and UVR8(W285A) formed a heterodimer via two distinct interfaces, designated Interface 1 and 2. The previously characterized Interface 1 is found between the RUP2 WD40 domain and the UVR8 C27 subregion. The newly identified Interface 2 is formed through interactions between the RUP2 WD40 domain and the UVR8 core domain. Disruption of Interface 2 impaired UV-B induced photomorphogenic development in Arabidopsis thaliana. Further biochemical analysis indicated that both interfaces are important for RUP2-UVR8 interactions and RUP2-mediated facilitation of UVR8 redimerization. Our findings suggest that the two-interface-interaction mode is adopted by both RUP2 and COP1 when they interact with UVR8, marking a step forward in understanding the molecular basis that underpins the interactions between UVR8 and its photocycle regulators.

引用

页数：12