Proteomic analysis of pulmonary arteries and lung tissues from dogs affected with pulmonary hypertension secondary to degenerative mitral valve disease

被引:2
|
作者
Sakarin, Siriwan [1 ]
Rungsipipat, Anudep [2 ]
Roytrakul, Sittiruk [3 ]
Jaresitthikunchai, Janthima [3 ]
Phaonakrop, Narumon [3 ]
Charoenlappanit, Sawanya [3 ]
Thaisakun, Siriwan [3 ]
Surachetpong, Sirilak Disatian [1 ]
机构
[1] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Med, Bangkok, Thailand
[2] Chulalongkorn Univ, Fac Vet Sci, Ctr Excellence Compan Anim Canc, Dept Pathol, Bangkok, Thailand
[3] Natl Ctr Genet Engn & Biotechnol BIOTEC, Funct Prote Technol Lab, Natl Sci & Technol Dev Agcy, Pathum Thani, Thailand
来源
PLOS ONE | 2024年 / 19卷 / 01期
关键词
HEPATOCYTE GROWTH-FACTOR; SMOOTH-MUSCLE-CELLS; PROTEIN; APOPTOSIS; HEART;
D O I
10.1371/journal.pone.0296068
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In dogs with degenerative mitral valve disease (DMVD), pulmonary hypertension (PH) is a common complication characterized by abnormally elevated pulmonary arterial pressure (PAP). Pulmonary arterial remodeling is the histopathological changes of pulmonary artery that has been recognized in PH. The underlying mechanisms that cause this arterial remodeling are poorly understood. This study aimed to perform shotgun proteomics to investigate changes in protein expression in pulmonary arteries and lung tissues of DMVD dogs with PH compared to normal control dogs and DMVD dogs without PH. Tissue samples were collected from the carcasses of 22 small-sized breed dogs and divided into three groups: control (n = 7), DMVD (n = 7) and DMVD+PH groups (n = 8). Differentially expressed proteins were identified, and top three upregulated and downregulated proteins in the pulmonary arteries of DMVD dogs with PH including SIK family kinase 3 (SIK3), Collagen type I alpha 1 chain (COL1A1), Transforming growth factor alpha (TGF-alpha), Apoptosis associated tyrosine kinase (AATYK), Hepatocyte growth factor activator (HGFA) and Tyrosine-protein phosphatase non-receptor type 13 (PTPN13) were chosen. Results showed that some of the identified proteins may play a role in the pathogenesis of pulmonary arterial remodeling. This study concluded shotgun proteomics has potential as a tool for exploring candidate proteins associated with the pathogenesis of PH secondary to DMVD in dogs.
引用
收藏
页数:18
相关论文
共 50 条
  • [21] Prevalence and Prognostic Importance of Pulmonary Hypertension in Dogs with Myxomatous Mitral Valve Disease
    Borgarelli, M.
    Abbott, J.
    Braz-Ruivo, L.
    Chiavegato, D.
    Crosara, S.
    Lamb, K.
    Ljungvall, I.
    Poggi, M.
    Santilli, R. A.
    Haggstrom, J.
    JOURNAL OF VETERINARY INTERNAL MEDICINE, 2015, 29 (02): : 569 - 574
  • [22] Pulmonary Hypertension in Aortic and Mitral Valve Disease
    Maeder, Micha T.
    Weber, Lukas
    Buser, Marc
    Gerhard, Marc
    Haager, Philipp K.
    Maisano, Francesco
    Rickli, Hans
    FRONTIERS IN CARDIOVASCULAR MEDICINE, 2018, 5
  • [23] Pulmonary Vascular Resistance Estimated by Echocardiography in Dogs With Myxomatous Mitral Valve Disease and Pulmonary Hypertension Probability
    Suzuki, Ryohei
    Yuchi, Yunosuke
    Kanno, Haruka
    Saito, Takahiro
    Teshima, Takahiro
    Matsumoto, Hirotaka
    Koyama, Hidekazu
    FRONTIERS IN VETERINARY SCIENCE, 2021, 8
  • [24] Prognostic value of pulmonary vascular resistance estimated by echocardiography in dogs with myxomatous mitral valve disease and pulmonary hypertension
    Yuchi, Yunosuke
    Suzuki, Ryohei
    Yasumura, Yuyo
    Saito, Takahiro
    Teshima, Takahiro
    Matsumoto, Hirotaka
    Koyama, Hidekazu
    JOURNAL OF VETERINARY INTERNAL MEDICINE, 2023, 37 (03) : 856 - 865
  • [25] Pulmonary Hypertension Secondary to Myxomatous Mitral Valve Disease in Dogs: Current Insights into the Histological Manifestation and Its Determining Factors
    Grzeczka, Arkadiusz
    Paslawska, Urszula
    Graczyk, Szymon
    Antosik, Paulina
    Zawadzki, Marcin
    Paslawski, Robert
    APPLIED SCIENCES-BASEL, 2024, 14 (06):
  • [26] Pulmonary Vein-to-Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease
    Merveille, A. -C.
    Bolen, G.
    Krafft, E.
    Roels, E.
    Gomart, S.
    Etienne, A. -L.
    Clercx, C.
    Mc Entee, K.
    JOURNAL OF VETERINARY INTERNAL MEDICINE, 2015, 29 (06): : 1502 - 1509
  • [27] PULMONARY PRECAPILLARY HYPERTENSION IN MITRAL-VALVE DISEASE
    WIDIMSKY, J
    COR ET VASA, 1983, 25 (01) : 17 - 27
  • [28] Denervation of Pulmonary Arteries in Patients with Mitral Valve Defects Complicated by Atrial Fibrillation and Pulmonary Hypertension
    Trofimov, N. A.
    Medvedev, A. P.
    Nikolsky, A., V
    Kichigin, V. A.
    Zhamlikhanova, S. S.
    Babokin, V. E.
    SOVREMENNYE TEHNOLOGII V MEDICINE, 2019, 11 (04) : 95 - 103
  • [29] Perivascular inflammatory cells and their association with pulmonary arterial remodelling in dogs with pulmonary hypertension due to myxomatous mitral valve disease
    Sakarin, Siriwan
    Rungsipipat, Anudep
    Surachetpong, Sirilak Disatian
    VETERINARY RESEARCH COMMUNICATIONS, 2023, 47 (03) : 1505 - 1521
  • [30] Perivascular inflammatory cells and their association with pulmonary arterial remodelling in dogs with pulmonary hypertension due to myxomatous mitral valve disease
    Siriwan Sakarin
    Anudep Rungsipipat
    Sirilak Disatian Surachetpong
    Veterinary Research Communications, 2023, 47 : 1505 - 1521