Metal-Organic Framework (UiO-66)-Delivered Phycocyanobilin

Phycocyanin (C-PC) is a constitutive chromoprotein of Arthrospira platensis, which exhibits promising efficacy against different types of cancer. In this study, we cleaved C-PC's chromophore phycocyanobilin (PCB) and demonstrated its ability as an anti-cancer drug for Colorectal cancer (CRC). PCB displayed an anti-cancer effect for CRC (HT-29) cells with IC50 of 108 mg/ml. Assessing the transcripts levels of some biomarkers revealed that the PCB caused an upregulation in the anti-metastatic gene NME1 level and downregulation of the COX -2 level. The flow cytometric results showed the effect of PCB on the arrest of the cell cycle's G1 phase. In addi-tion, we successfully synthesized the UiO-66 (Zr-MOF). We incorporated the PCB into UiO-66 nanoparticles with a loading percentage of 46 %. Assessment of the cytotoxic effects of UiO-66@PCB showed a 2-fold improvement in the IC50 compared to the free PCB. In conclusion, we have shown that PCB displayed a prom-ising potential as an anti-cancer agent. Yet, it is considered a safe and natural substance that can help to miti-gate cancer spread and symptoms. In the meantime, UiO-66 can be used as a safe nano-delivery tool for PCB.(c) 2022 Published by Elsevier Inc. on behalf of American Pharmacists Association.