High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9

被引：6
|
作者：
Chen, Yudong [1 ]
Xiang, Haiying [1 ]
Jia, Ling [2 ]
Yang, Qianxu [1 ]
Zhang, Jianduo [1 ]
Jiang, Jiarui [1 ]
Zeng, Wanli [1 ]
Deng, Lele [1 ]
Jin, Jingjing [3 ]
Gao, Qian [1 ]
Li, Xuemei [1 ]
机构：
[1] Yunnan Acad Tobacco Sci, Kunming 650106, Peoples R China
[2] Southwest Univ, Biol Sci Res Ctr, Chongqing 400715, Peoples R China
[3] Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China
来源：
PLANT CELL REPORTS | 2023年 / 42卷 / 12期
关键词：
D O I：
10.1007/s00299-023-03050-5
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Key message4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
引用
收藏
页码：2039 / 2042
页数：4
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