Urine soluble TLR4 levels may contribute to predict urinary tract infection in children: the UTILISE Study

被引：1

作者：

Aksu, Bagdagul ^{[1
,2
]}

Afonso, Alberto Caldas ^{[3
]}

Akil, Ipek ^{[4
]}

Alpay, Harika ^{[5
]}

Atmis, Bahriye ^{[6
,7
]}

Aydog, Ozlem ^{[8
]}

Bayazit, Aysun Karabay ^{[7
]}

Bayram, Meral Torun ^{[9
]}

Bilge, Ilmay ^{[10
]}

Bulut, Ipek Kaplan ^{[11
]}

Buyukkaragoz, Bahar ^{[12
]}

Comak, Elif ^{[13
]}

Demir, Belde Kasap ^{[14
,15
]}

Dincel, Nida ^{[16
]}

Donmez, Osman ^{[17
]}

Durmus, Mehmet Akif ^{[18
]}

Dursun, Hasan ^{[19
]}

Dusunsel, Ruhan ^{[20
]}

Duzova, Ali ^{[21
]}

Ertan, Pelin ^{[4
]}

Gedikbasi, Asuman ^{[22
]}

Goknar, Nilufer ^{[23
]}

Guven, Sercin ^{[5
]}

Hacihamdioglu, Duygu ^{[24
]}

Jankauskiene, Augustina ^{[25
]}

Kalyoncu, Mukaddes ^{[26
]}

Kavukcu, Salih ^{[9
]}

Kenan, Bahriye Uzun ^{[12
]}

Kucuk, Nuran ^{[27
]}

Kural, Bahar ^{[28
]}

Litwin, Mieczyslaw ^{[29
]}

Montini, Giovanni ^{[30
,31
]}

Morello, William ^{[30
]}

Obrycki, Lukasz ^{[29
]}

Omer, Beyhan ^{[32
]}

Oner, Huseyin Adil ^{[2
]}

Ozdemir, Ebru Misirli ^{[33
]}

Ozkayin, Nese ^{[34
]}

Paripovic, Dusan ^{[35
]}

Pehlivanoglu, Cemile ^{[36
]}

Saygili, Seha ^{[37
]}

Schaefer, Franz ^{[38
]}

Schaefer, Susanne ^{[38
]}

Sonmez, Ferah ^{[39
]}

Tabel, Yilmaz ^{[40
]}

Tas, Nesrin ^{[21
]}

Tasdemir, Mehmet ^{[10
,41
]}

Teixeira, Ana ^{[3
]}

Tekcan, Demet ^{[8
]}

Topaloglu, Rezan ^{[21
]}

机构：

[1] Istanbul Univ, Inst Child Hlth, Dept Pediat Basic Sci, Istanbul, Turkiye

[2] Istanbul Univ, Istanbul Fac Med, Div Pediat Nephrol, Istanbul, Turkiye

[3] Univ Porto, Ctr Hospitalar, Div Pediat Nephrol, Ctr Materno Infantil Norte, Porto, Portugal

[4] Celal Bayar Univ, Fac Med, Div Pediat Nephrol, Manisa, Turkiye

[5] Marmara Univ, Fac Med, Div Pediat Nephrol, Istanbul, Turkiye

[6] Erzurum Training & Res Hosp, Pediat Nephrol, Erzurum, Turkiye

[7] Cukurova Univ, Fac Med, Div Pediat Nephrol, Adana, Turkiye

[8] Ondokuz Mayis Univ, Fac Med, Div Pediat Nephrol, Samsun, Turkiye

[9] Dokuz Eylul Univ, Fac Med, Div Pediat Nephrol, Izmir, Turkiye

[10] Koc Univ, Div Pediat Nephrol, Sch Med, Dept Pediat, Istanbul, Turkiye

[11] Ege Univ, Fac Med, Div Pediat Nephrol, Izmir, Turkiye

[12] Gazi Univ, Fac Med, Div Pediat Nephrol, Ankara, Turkiye

[13] Akdeniz Univ, Fac Med, Div Pediat Nephrol, Antalya, Turkiye

[14] Univ Hlth Sci, Tepecik Training & Res Hosp, Div Pediat Nephrol, Izmir, Turkiye

[15] Izmir Katip Celebi Univ, Fac Med, Div Pediat Nephrol, Izmir, Turkiye

[16] Univ Hlth Sci, Dr Behcet Uz Children Dis Training & Res Hosp, Div Pediat Nephrol, Izmir, Turkiye

[17] Uludag Univ, Fac Med, Div Pediat Nephrol, Bursa, Turkiye

[18] Istanbul Univ, Istanbul Fac Med, Dept Med Microbiol, Istanbul, Turkiye

[19] Univ Hlth Sci, Okmeydani Training & Res Hosp, Div Pediat Nephrol, Istanbul, Turkiye

[20] Erciyes Univ, Fac Med, Div Pediat Nephrol, Kayseri, Turkiye

[21] Hacettepe Univ, Fac Med, Div Pediat Nephrol, Ankara, Turkiye

[22] Istanbul Univ, Inst Child Hlth, Dept Rare Dis, Istanbul, Turkiye

[23] Univ Hlth Sci, Bagcilar Training & Res Hosp, Div Pediat Nephrol, Istanbul, Turkiye

[24] Bahcesehir Univ, Fac Med, Div Pediat Nephrol, Istanbul, Turkiye

[25] Vilnius Univ, Inst Clin Med, Clin Pediat, Vilnius, Lithuania

[26] Karadeniz Tech Univ, Fac Med, Div Pediat Nephrol, Trabzon, Turkiye

[27] Kartal Training & Res Hosp, Div Pediat Nephrol, Istanbul, Turkiye

[28] Hlth Sci Univ Bakirkoy Sadi Konuk Training, Res Hosp, Dept Pediat, Istanbul, Turkiye

[29] Childrens Mem Hlth Inst, Div Nephrol, Kidney Transplantat & Hypertens, Warsaw, Poland

[30] Osped Maggiore Policlin, Fdn IRCCS CaGranda, Pediat Nephrol Dialysis & Transplant Unit, Milan, Italy

[31] Univ Milan, Dept Clin Sci & Community Hlth, Milan, Italy

[32] Istanbul Univ, Istanbul Fac Med, Dept Biochem, Istanbul, Turkiye

[33] Okmeydani Training & Res Hosp, Dept Pediat, Istanbul, Turkiye

[34] Trakya Univ, Fac Med, Div Pediat Nephrol, Edirne, Turkiye

[35] Univ Childrens Hosp, Div Pediat Nephrol, Belgrade, Serbia

[36] Umraniye Training & Res Hosp, Div Pediat Nephrol, Istanbul, Turkiye

[37] Istanbul Univ Cerrahpasa, Cerrahpasa Fac Med, Div Pediat Nephrol, Istanbul, Turkiye

[38] Heidelberg Univ, Ctr Pediat & Adolescent Med, Div Pediat Nephrol, Heidelberg, Germany

[39] Adnan Menderes Univ, Fac Med, Div Pediat Nephrol, Aydin, Turkiye

[40] Inonu Univ, Fac Med, Div Pediat Nephrol, Malatya, Turkiye

[41] Istinye Univ, Liv Hosp Ulus, Fac Med, Dept Pediat Nephrol, Istanbul, Turkiye

[42] Bakirkoy Dr Sadi Konuk Training & Res Hosp, Div Pediat Nephrol, Istanbul, Turkiye

[43] Dortcelik Childrens Hosp, Div Pediat Nephrol, Bursa, Turkiye

[44] Bursa City Hosp, Div Pediat Nephrol, Bursa, Turkiye

[45] Istanbul Univ, Istanbul Fac Med, Div Pediat Emergency, Istanbul, Turkiye

[46] Univ Hlth Sci, Kanuni Sultan Suleyman Res & Training Hosp, Dept Pediat, Div Pediat Nephrol, Istanbul, Turkiye

[47] Univ Hlth Sci, Kanuni Sultan Suleyman Res & Training Hosp, Dept Pediat, Istanbul, Turkiye

[48] Pamukkale Univ, Fac Med, Div Pediat Nephrol, Denizli, Turkiye

[49] Istanbul Univ, Istanbul Fac Med, Dept Biostat, Istanbul, Turkiye

来源：

PEDIATRIC NEPHROLOGY | 2024年 / 39卷 / 02期

关键词：

Toll-like receptor 4; TLR4; Urinary tract infection; UTI; UTILISE study; INTERLEUKIN-8; EXPRESSION; UTI;

D O I：

10.1007/s00467-023-06063-0

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Background One of the most common bacterial infections in childhood is urinary tract infection (UTI). Toll-like receptors (TLRs) contribute to immune response against UTI recognizing specific pathogenic agents. Our aim was to determine whether soluble TLR4 (sTLR4), soluble TLR5 (sTLR5) and interleukin 8 (IL-8) can be used as biomarkers to diagnose UTI. We also aimed to reveal the relationship between urine Heat Shock Protein 70 (uHSP70) and those biomarkers investigated in this study.Methods A total of 802 children from 37 centers participated in the study. The participants (n = 282) who did not meet the inclusion criteria were excluded from the study. The remaining 520 children, including 191 patients with UTI, 178 patients with non-UTI infections, 50 children with contaminated urine samples, 26 participants with asymptomatic bacteriuria and 75 healthy controls were included in the study. Urine and serum levels of sTLR4, sTLR5 and IL-8 were measured at presentation in all patients and after antibiotic treatment in patients with UTI.Results Urine sTLR4 was higher in the UTI group than in the other groups. UTI may be predicted using 1.28 ng/mL as cut-off for urine sTLR4 with 68% sensitivity and 65% specificity (AUC = 0.682). In the UTI group, urine sTLR4 levels were significantly higher in pyelonephritis than in cystitis (p < 0.0001). Post-treatment urine sTLR4 levels in the UTI group were significantly lower than pre-treatment values (p < 0.0001).Conclusions Urine sTLR4 may be used as a useful biomarker in predicting UTI and subsequent pyelonephritis in children with UTI.

引用

页码：483 / 491

页数：9

共 39 条

[1] Urine soluble TLR4 levels may contribute to predict urinary tract infection in children: the UTILISE Study
Bagdagul Aksu
Alberto Caldas Afonso
Ipek Akil
Harika Alpay
Bahriye Atmis
Ozlem Aydog
Aysun Karabay Bayazıt
Meral Torun Bayram
Ilmay Bilge
Ipek Kaplan Bulut
Bahar Buyukkaragoz
Elif Comak
Belde Kasap Demir
Nida Dincel
Osman Donmez
Mehmet Akif Durmus
Hasan Dursun
Ruhan Dusunsel
Ali Duzova
Pelin Ertan
Asuman Gedikbasi
Nilufer Goknar
Sercin Guven
Duygu Hacihamdioglu
Augustina Jankauskiene
Mukaddes Kalyoncu
Salih Kavukcu
Bahriye Uzun Kenan
Nuran Kucuk
Bahar Kural
Mieczysław Litwin
Giovanni Montini
William Morello
Lukasz Obrycki
Beyhan Omer
Huseyin Adil Oner
Ebru Misirli Ozdemir
Nese Ozkayin
Dusan Paripovic
Cemile Pehlivanoglu
Seha Saygili
Franz Schaefer
Susanne Schaefer
Ferah Sonmez
Yilmaz Tabel
Nesrin Tas
Mehmet Tasdemir
Ana Teixeira
Demet Tekcan
Rezan Topaloglu
Pediatric Nephrology, 2024, 39 : 483 - 491
[2] Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
Yilmaz, Alev
Afonso, Alberto Caldas
Akil, Ipek
Aksu, Bagdagul
Alpay, Harika
Atmis, Bahriye
Aydog, Ozlem
Bayram, Meral Torun
Bilge, Ilmay
Bulut, Ipek Kaplan
Buyukkaragoz, Bahar
Comak, Elif
Demir, Belde Kasap
Dincel, Nida
Donmez, Osman
Durmus, Mehmet Akif
Dursun, Hasan
Dusunsel, Ruhan
Duzova, Ali
Ertan, Pelin
Gedikbasi, Asuman
Goknar, Nilufer
Guven, Sercin
Hacihamdioglu, Duygu
Jankauskiene, Augustina
Kalyoncu, Mukaddes
Kavukcu, Salih
Kenan, Bahriye Uzun
Kucuk, Nuran
Kural, Bahar
Montini, Giovanni
Morello, William
Nayir, Ahmet
Obrycki, Lukasz
Omer, Beyhan
Ozdemir, Ebru Misirli
Ozkayin, Nese
Paripovic, Dusan
Pehlivanoglu, Cemile
Saygili, Seha
Schaefer, Susanne
Sonmez, Ferah
Tabel, Yilmaz
Tas, Nesrin
Tasdemir, Mehmet
Teixeira, Ana
Tekcan, Demet
Tulpar, Sebahat
Turkkan, Ozde Nisa
Uysal, Berfin
PEDIATRIC NEPHROLOGY, 2023, 38 (03) : 791 - 799
[3] URINARY HSP70 IMPROVES DIAGNOSTIC ACCURACY FOR URINARY TRACT INFECTION IN CHILDREN: UTILISE STUDY
Yilmaz, Alev
Afonso, Alberto Caldas
Akil, Ipek
Aksu, Bagdagul
Alpay, Harika
Atmis, Bahriye
Aydog, Ozlem
Bayazit, Aysun Karabay
Bayram, Meral Torun
Bilge, Ilmay
Bulut, Ipek Kaplan
Buyukkaragoz, Bahar
Comak, Elif
Demir, Belde Kasap
Dincel, Nida
Donmez, Osman
Durmus, Mehmet Akif
Dursun, Hasan
Dusunsel, Ruhan
Duzova, Ali
Ertan, Pelin
Gedikbasi, Asuman
Goknar, Nilufer
Guven, Sercin
Hacihamdioglu, Duygu
Jankauskiene, Augustina
Kalyoncu, Mukaddes
Kavukcu, Salih
Kenan, Bahriye Uzun
Kucuk, Nuran
Kural, Bahar
Litwin, Mieczyslaw
Montini, Giovanni
Morello, William
Nayir, Ahmet
Obrycki, Lukasz
Omer, Beyhan
Ozdemir, Ebru Misirli
Ozkayin, Nese
Paripovic, Dusan
Pehlivanoglu, Cemile
Saygili, Seha
Schaefer, Susanne
Sonmez, Ferah
Tabel, Yilmaz
Tas, Nesrin
Tasdemir, Mehmet
Teixeira, Ana
Tekcan, Demet
Tulpar, Sebahat
PEDIATRIC NEPHROLOGY, 2022, 37 (11) : 2807 - 2808
[4] Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
Alev Yilmaz
Alberto Caldas Afonso
Ipek Akil
Bagdagul Aksu
Harika Alpay
Bahriye Atmis
Ozlem Aydog
Aysun Karabay Bayazıt
Meral Torun Bayram
Ilmay Bilge
Ipek Kaplan Bulut
Bahar Buyukkaragoz
Elif Comak
Belde Kasap Demir
Nida Dincel
Osman Donmez
Mehmet Akif Durmus
Hasan Dursun
Ruhan Dusunsel
Ali Duzova
Pelin Ertan
Asuman Gedikbasi
Nilufer Goknar
Sercin Guven
Duygu Hacihamdioglu
Augustina Jankauskiene
Mukaddes Kalyoncu
Salih Kavukcu
Bahriye Uzun Kenan
Nuran Kucuk
Bahar Kural
Mieczysław Litwin
Giovanni Montini
William Morello
Ahmet Nayir
Lukasz Obrycki
Beyhan Omer
Ebru Misirli Ozdemir
Nese Ozkayin
Dusan Paripovic
Cemile Pehlivanoglu
Seha Saygili
Susanne Schaefer
Ferah Sonmez
Yilmaz Tabel
Nesrin Tas
Mehmet Tasdemir
Ana Teixeira
Demet Tekcan
Sebahat Tulpar
Pediatric Nephrology, 2023, 38 : 791 - 799
[5] Altered Expression of TLR2 and TLR4 on Peripheral CD14+Blood Monocytes in Children with Urinary Tract Infection
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PLOS ONE, 2010, 5 (05):
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[8] LY96, UPKIB Mutations and TLR4, CD14, MBL Polymorphisms in Children with Urinary Tract Infection
Pelin Ertan
Afig Berdeli
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The Indian Journal of Pediatrics, 2011, 78 : 1229 - 1233
[9] LY96, UPKIB Mutations and TLR4, CD14, MBL Polymorphisms in Children with Urinary Tract Infection
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