In vitro expansion of Wilms' tumor protein 1 epitope- specific primary T cells from healthy human peripheral blood mononuclear cells

被引：1

作者：

Heijden, Sanne van der ^{[1
,2
]}

Flumens, Donovan ^{[1
]}

Versteven, Maarten ^{[1
]}

Peeters, Stefanie ^{[1
]}

De Reu, Hans ^{[1
]}

Campillo-Davo, Diana ^{[1
]}

Willemen, Yannick ^{[1
]}

Ogunjimi, Benson ^{[4
]}

Van Tendeloo, Viggo ^{[1
]}

Berneman, Zwi N. ^{[1
]}

Anguille, Sebastien ^{[1
,3
]}

Smits, Evelien ^{[2
]}

Lion, Eva ^{[1
]}

机构：

[1] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, Fac Med & Hlth Sci, Lab Expt Hematol LEH, Univplein 1, B-2610 Antwerp, Belgium

[2] Univ Antwerp, Ctr Oncol Res CORE, Integrated Personalized & Precis Oncol Network IPP, Univplein 1, B-2610 Antwerp, Belgium

[3] Antwerp Univ Hosp UZA, Div Hematol, Drie Eikenstr 655, B-2650 Edegem, Belgium

[4] Univ Antwerp, Ctr Hlth Econ Res & Modeling Infect Dis CHERMID, VAXINFECTIO, Univplein 1, B-2610 Antwerp, Belgium

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cancer; Cell Biology; Cell Culture; Cell Isolation; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology;

D O I：

10.1016/j.xpro.2023.102053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Wilms' tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT137-45-specific TCRs using healthy human peripheral blood mononuclear cells. We describe the expansion of WT1-specific T cell clones by two consecutive in vitro stimulations with autologous WT137-45-pulsed dendritic cells and peripheral blood lymphocytes. We then detail the detection with human leukocyte antigen/WT137-45 tetramers.

引用

页数：18

共 50 条

[21] Proangiogenic Cell Colonies Grown In Vitro from Human Peripheral Blood Mononuclear Cells
Mavromatis, Kreton
Sutcliffe, Diane J.
Joseph, Giji
Alexander, R. Wayne
Waller, Edmund K.
Quyyumi, Arshed A.
Taylor, W. Robert
JOURNAL OF BIOMOLECULAR SCREENING, 2012, 17 (09) : 1128 - 1135
[22] In Vitro Immunization of Human Peripheral Blood Mononuclear Cells (PBMC) Using Targeted Activated T Cells to Induce Specific Primary Humoral Antibody Responses Against Breast Cancer
Thakia, Archana
Norkina, Oxana
Lum, Lawrence G.
JOURNAL OF IMMUNOTHERAPY, 2009, 32 (09) : 963 - 963
[23] BMI1 enables extensive expansion of functional erythroblasts from human peripheral blood mononuclear cells
Liu, Senquan
Wu, Mengyao
Lancelot, Moira
Deng, Jiusheng
Gao, Yongxing
Roback, John D.
Chen, Tong
Cheng, Linzhao
MOLECULAR THERAPY, 2021, 29 (05) : 1918 - 1932
[24] A Cell-Free Protein Expression System Derived from Human Primary Peripheral Blood Mononuclear Cells
Burgenson, David
Linton, Jonathan
Ge, Xudong
Kostov, Yordan
Tolosa, Leah
Szeto, Gregory L.
Rao, Govind
ACS SYNTHETIC BIOLOGY, 2020, 9 (08): : 2188 - 2196
[25] The effect of catechol on human peripheral blood mononuclear cells (in vitro study)
Bukowska, Bozena
Michalowicz, Jaromir
Marczak, Agnieszka
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2015, 39 (01) : 187 - 193
[26] In vitro proliferative response of human peripheral blood mononuclear cells to concanavalin A
Lijnen, P
Saavedra, A
Petrov, V
CLINICA CHIMICA ACTA, 1997, 264 (01) : 91 - 101
[27] Human fetal but not adult normal mononuclear blood cells express the Wilms' tumor gene
Menssen, HD
Renkl, HJ
Entezami, M
Komischke, B
Schwartz, S
Rodeck, U
Thiel, E
BLOOD, 1995, 86 (10) : 2686 - 2686
[28] In vitro effect of lupeol and casearin G on peripheral blood mononuclear and tumor cells
Dupuy, Omar A.
Bonilla, Jose A., V
Murillo, Renato
Taylor, Peter
Jesus Abad, Maria
Gonzalez, Lorena
Juliao A, Johanna
REVISTA MEDICA DE CHILE, 2013, 141 (09) : 1150 - 1157
[29] In Vitro Determination of the Immunogenic Impact of Nanomaterials on Primary Peripheral Blood Mononuclear Cells
David, Christopher A. W.
Barrow, Michael
Murray, Patricia
Rosseinsky, Matthew J.
Owen, Andrew
Liptrott, Neill J.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (16) : 1 - 16
[30] Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Kondo, Makoto
Izumi, Takamichi
Fujieda, Nao
Kondo, Atsushi
Morishita, Takeharu
Matsushita, Hirokazu
Kakimi, Kazuhiro
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2011, (55):

← 1 2 3 4 5 →