In vitro expansion of Wilms' tumor protein 1 epitope- specific primary T cells from healthy human peripheral blood mononuclear cells

被引：1

作者：

Heijden, Sanne van der ^{[1
,2
]}

Flumens, Donovan ^{[1
]}

Versteven, Maarten ^{[1
]}

Peeters, Stefanie ^{[1
]}

De Reu, Hans ^{[1
]}

Campillo-Davo, Diana ^{[1
]}

Willemen, Yannick ^{[1
]}

Ogunjimi, Benson ^{[4
]}

Van Tendeloo, Viggo ^{[1
]}

Berneman, Zwi N. ^{[1
]}

Anguille, Sebastien ^{[1
,3
]}

Smits, Evelien ^{[2
]}

Lion, Eva ^{[1
]}

机构：

[1] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, Fac Med & Hlth Sci, Lab Expt Hematol LEH, Univplein 1, B-2610 Antwerp, Belgium

[2] Univ Antwerp, Ctr Oncol Res CORE, Integrated Personalized & Precis Oncol Network IPP, Univplein 1, B-2610 Antwerp, Belgium

[3] Antwerp Univ Hosp UZA, Div Hematol, Drie Eikenstr 655, B-2650 Edegem, Belgium

[4] Univ Antwerp, Ctr Hlth Econ Res & Modeling Infect Dis CHERMID, VAXINFECTIO, Univplein 1, B-2610 Antwerp, Belgium

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cancer; Cell Biology; Cell Culture; Cell Isolation; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology;

D O I：

10.1016/j.xpro.2023.102053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Wilms' tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT137-45-specific TCRs using healthy human peripheral blood mononuclear cells. We describe the expansion of WT1-specific T cell clones by two consecutive in vitro stimulations with autologous WT137-45-pulsed dendritic cells and peripheral blood lymphocytes. We then detail the detection with human leukocyte antigen/WT137-45 tetramers.

引用

页数：18

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