Behavior of breast cancer cells under oxygen concentration gradients in a microfluidic device

被引：0

作者：

Aratake, Satoshi ^{[1
]}

Kawahara, Naoto ^{[1
]}

Funamoto, Kenichi ^{[2
]}

机构：

[1] Tohoku Univ, Grad Sch Biomed Engn, Sendai, Miyagi, Japan

[2] Tohoku Univ, Inst Fluid Sci, Sendai, Miyagi, Japan

来源：

2023 45TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY, EMBC | 2023年

关键词：

CULTURE;

D O I：

10.1109/EMBC40787.2023.10340327

中图分类号：

TP18 [人工智能理论];

学科分类号：

081104 ; 0812 ; 0835 ; 1405 ;

摘要：

The tumor microenvironment (TME) is known as a chronic hypoxic environment, with spatiotemporal variation in oxygen concentration depending on the distance from blood vessels and the blood supply. In our previous studies, cancer cell behavior was observed under hypoxic conditions with spatial variation of oxygen concentration (oxygen concentration gradients); however, that under oxygen concentration gradients at low oxygen levels found in the TME has not been studied. In this study, we investigated the behavior of breast cancer cells at various oxygen concentration gradients, generated using a microfluidic device with oxygen concentration controllability. The results showed that cell distribution was altered in response to oxygen concentration, and tended to increase in a specific region at around 5% O-2. Evaluation of changes in cell numbers due to proliferation, migration, and cell death indicated that proliferation strongly affected cell distribution.

引用

页数：4

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