Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

被引：4
|
作者：
Urata, Shinji [1 ]
Okabe, Shigeo [2 ]
机构：
[1] Univ Tokyo, Grad Sch Med, Dept Otolaryngol, Tokyo 1130033, Japan
[2] Univ Tokyo, Grad Sch Med, Dept Cellular Neurobiol, Tokyo, Japan
来源：
ANATOMICAL SCIENCE INTERNATIONAL | 2023年 / 98卷 / 03期
关键词：
Stria vascularis; Cochlea; Optical tissue clearing; ScaleS; Macrophage; STRIA VASCULARIS; WHOLE-BRAIN; CELL; QUANTIFICATION; ACTIVATION; BARRIER; ORGANS;
D O I：
10.1007/s12565-023-00703-z
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1(+/GFP) mice. This study's method was effective in elucidating inner ear function under both physiological and pathological conditions.
引用
收藏
页码：309 / 317
页数：9
相关论文
共 50 条
  • [41] Three-dimensional reconstructions from optical sections of thick mouse inner ears using confocal microscopy
    Kopecky, B. J.
    Duncan, J. S.
    Elliott, K. L.
    Fritzsch, B.
    JOURNAL OF MICROSCOPY, 2012, 248 (03) : 292 - 298
  • [42] Optimizing Imaging of Three-Dimensional Multicellular Tumor Spheroids with Fluorescent Reporter Proteins Using Confocal Microscopy
    le Roux, Lucia
    Volgin, Andrei
    Maxwell, David
    Ishihara, Katashi
    Gelovani, Juri
    Schellingerhout, Dawid
    MOLECULAR IMAGING, 2008, 7 (05): : 214 - 221
  • [43] CSI Helsinki: comparing three-dimensional imaging of diagonal cutter toolmarks using confocal microscopy and SWLI
    Kassamakov, I.
    Barbeau, C.
    Lehto, S.
    Ahvenainen, P.
    Reinikainen, T.
    Haeggstrom, E.
    THREE-DIMENSIONAL IMAGING, VISUALIZATION, AND DISPLAY 2010 AND DISPLAY TECHNOLOGIES AND APPLICATIONS FOR DEFENSE, SECURITY, AND AVIONICS IV, 2010, 7690
  • [44] Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy
    Liu, Yanyan
    Yanagishima, Taiki
    Curran, Arran
    Edmond, Kazem V.
    Sacanna, Stefano
    Dullens, Roel P. A.
    LANGMUIR, 2019, 35 (24) : 7962 - 7969
  • [45] THREE-DIMENSIONAL MICROSCOPY OF BIOPSIES WITH A HANDHELD CONFOCAL MICROSCOPE
    Piyawattanametha, Wibool
    2015 INTERNATIONAL CONFERENCE ON OPTICAL MEMS AND NANOPHOTONICS (OMN), 2015,
  • [46] THREE-DIMENSIONAL ALIGNMENT AND MERGING OF CONFOCAL MICROSCOPY STACKS
    Ramesh, Nisha
    Otsuna, Hideo
    Tasdizen, Tolga
    2013 20TH IEEE INTERNATIONAL CONFERENCE ON IMAGE PROCESSING (ICIP 2013), 2013, : 1447 - 1450
  • [47] Three-dimensional confocal reflectance microscopy for surface metrology
    Kim, Chang-Soo
    Yoo, Hongki
    MEASUREMENT SCIENCE AND TECHNOLOGY, 2021, 32 (10)
  • [48] Three-dimensional confocal microscopy of the human cornea in vivo
    Masters, BR
    Böhnke, M
    OPHTHALMIC RESEARCH, 2001, 33 (03) : 125 - 135
  • [49] Three-Dimensional Tracking of Fluorescent Particles in Confocal Microscopy
    Ashley, Trevor
    Chan-Tse, Catherine
    Worthey, Jeffrey
    Andersson, Sean
    BIOPHYSICAL JOURNAL, 2013, 104 (02) : 525A - 525A
  • [50] Three-dimensional Bayesian image analysis and confocal microscopy
    Al-Awadhi, Fahimah
    Hurn, Merrilee
    Jennison, Christopher
    JOURNAL OF APPLIED STATISTICS, 2011, 38 (01) : 29 - 46
12345