FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation

被引：7

作者：

Saraeva, Irina ^{[1
]}

Tolordava, Eteri ^{[1
,2
]}

Sheligyna, Svetlana ^{[1
]}

Nastulyavichus, Alyona ^{[1
]}

Khmelnitskii, Roman ^{[1
]}

Pokryshkin, Nikolay ^{[3
]}

Khmelenin, Dmitriy ^{[4
]}

Kudryashov, Sergey ^{[1
,5
]}

Ionin, Andrey ^{[1
]}

Akhmatkhanov, Andrey ^{[5
]}

机构：

[1] Russian Acad Sci, PN Lebedev Phys Inst, Moscow 119991, Russia

[2] NF Gamaleya Fed Res Ctr Epidemiol & Microbiol, Moscow 123098, Russia

[3] Moscow MV Lomonosov State Univ, Fac Phys, Moscow 119991, Russia

[4] Russian Acad Sci, Inst Crystallog, Branch Fed Sci Res Ctr Crystallog & Photon, Moscow 119333, Russia

[5] Ural Fed Univ, Sch Nat Sci & Math, Ekaterinburg 620000, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 06期

关键词：

Fourier-transform IR spectroscopy; IR laser inactivation; bacteria; ER-YAG LASER; INFRARED-SPECTROSCOPY; TRANSFORM; SPORES;

D O I：

10.3390/ijms24065119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 mu m and 6.04 mu m, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells in these spectral ranges: vibrations of amide groups in proteins (1500-1700 cm(-1)), and C-H vibrations in membrane proteins and lipids (2800-3000 cm(-1)). The underlying bactericidal structural molecular changes were revealed by the stationary Fourier-transform IR spectroscopy, with the spectral peaks parameters being obtained by Lorentzian fitting with the hidden peaks revealed by the second derivative calculations, while no visible damage to the cell membranes was identified by scanning and transmission electron microscopy.

引用

页数：10

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