Smartphone-based microplate reader for high-throughput quantitation of disease markers in serum

被引:8
|
作者
Deng, Rong [1 ]
Chao, Xiaoxin [1 ]
Li, Haiqin [1 ]
Li, Xiaochun [1 ]
Yang, Zehua [2 ]
Yu, Hua-Zhong [1 ,3 ]
机构
[1] Taiyuan Univ Technol, Inst Biomed Precis Testing & Instrumentat, Coll Biomed Engn, Yuci 030600, Shanxi, Peoples R China
[2] Shanxi Med Univ, Hosp 1, Med Lab, Taiyuan 030024, Shanxi, Peoples R China
[3] Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada
关键词
C-REACTIVE PROTEIN; LINKED-IMMUNOSORBENT-ASSAY; BIOMARKER; CANCER; BIOLOGY; ELISA;
D O I
10.1039/d2an01571d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Herein, a smartphone-based portable reader with integrated optics for standard microtiter plates (96 wells) has been designed and demonstrated for high-throughput quantitation of validated biomarkers in serum. The customized optical attachment was simply constructed with a convex lens and a light source, by which the transmitted light through a 96-well microtiter plate was converged for imaging with a smartphone, so that accurate and wide-range reading of the plate can be achieved. More importantly, relying on the digitized colorimetric analysis of the obtained images, concentrations of various biomarkers can be determined directly using the customized mobile app. A set of validated biomarkers for inflammation and infection, C-reactive protein (CRP), serum amyloid A (SAA), and procalcitonin (PCT) have been quantitated with this new system; both the response ranges and limits of detection meet the requirement of clinical tests. The consistency with the results obtained using a commercial microplate reader proves its reliability and precision, augments its potential as a point-of-care diagnostic device for on-site testing or resource-limited settings.
引用
收藏
页码:735 / 741
页数:8
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