Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female

被引：2
|
作者：
Nimonkar, Madhura Milind [1 ]
Arunachal, Gautham [2 ]
Dash, Suravi Sasmita [1 ]
Kandy, Swapna Kannothum [1 ]
Raghavendra, Kenchaiah [3 ]
Chetan, Ghati K. [2 ]
Mehta, Bhupesh [1 ]
Markandeya, Yogananda S. [1 ]
机构：
[1] Natl Inst Mental Hlth & Neurosci NIMHANS, Dept Biophys, Bengaluru, India
[2] Natl Inst Mental Hlth & Neuro Sci NIMHANS, Dept Human Genet, Bengaluru, Karnataka, India
[3] Natl Inst Mental Hlth & Neurosci NIMHANS, Dept Neurol, Bengaluru, India
来源：
STEM CELL RESEARCH | 2023年 / 70卷
关键词：
D O I：
10.1016/j.scr.2023.103130
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors-SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms.
引用
收藏
页数：4
相关论文
共 50 条
  • [41] Generation of two induced pluripotent stem cell lines from peripheral blood mononuclear cells of a patient with Wilson's disease
    Malakhova, A. A.
    Grigor'eva, E., V
    Vasilyeva, O. Yu
    Zhigalina, D., I
    Skryabin, N. A.
    Sivtcev, A. A.
    Kolesnikov, N. A.
    Bueverov, A. O.
    Lebedev, I. N.
    Bogomolov, P. O.
    Zakian, S. M.
    STEM CELL RESEARCH, 2020, 47
  • [42] Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Ross, Samantha Barratt
    Fraser, Stuart T.
    Bagnall, Richard D.
    Semsarian, Christopher
    STEM CELL RESEARCH, 2017, 20 : 76 - 79
  • [43] Generation of human induced pluripotent stem cell line from peripheral blood of patient with lymphedema-distichiasis syndrome
    Bialobrzeska, Marta
    Stepniewski, Jacek
    Martyniak, Alicja
    Szuba, Andrzej
    Dulak, Jozef
    STEM CELL RESEARCH, 2025, 85
  • [44] Induced pluripotent stem cells (iPSCs) derived from frontotemporal dementia patient's peripheral blood mononuclear cells
    Lee, Han-Kyu
    Morin, Peter
    Wells, John
    Hanlon, Eugene B.
    Xia, Weiming
    STEM CELL RESEARCH, 2015, 15 (02) : 325 - 327
  • [45] Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
    Li, Yueying
    Hai, Yong
    Chen, Jiayu
    Liu, Tie
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017, (125):
  • [46] Generation of Induced Pluripotent Stem Cells with High Efciency from Human Umbilical Cord Blood Mononuclear Cells
    Juan Wang
    Qi Gu
    Jie Hao
    Donghui Bai
    Lei Liu
    Xiaoyang Zhao
    Zhonghua Liu
    Liu Wang
    Qi Zhou
    Genomics,Proteomics & Bioinformatics, 2013, 11 (05) : 304 - 311
  • [47] Generation of Induced Pluripotent Stem Cells with High Efciency from Human Umbilical Cord Blood Mononuclear Cells
    Juan Wang
    Qi Gu
    Jie Hao
    Donghui Bai
    Lei Liu
    Xiaoyang Zhao
    Zhonghua Liu
    Liu Wang
    Qi Zhou
    Genomics,Proteomics & Bioinformatics, 2013, (05) : 304 - 311
  • [48] Induction of stem cell-like plasticity in mononuclear cells derived from unmobilised adult human peripheral blood
    Abuljadayel, HS
    CURRENT MEDICAL RESEARCH AND OPINION, 2003, 19 (05) : 355 - 375
  • [49] Generation of an induced pluripotent stem cell line from a healthy Caucasian male
    Martinez-Moreno, Rebecca
    Perez-Serra, Alexandra
    Carreras, David
    Aran, Begona
    Kuebler, Bernd
    Brugada, Ramon
    Scornik, Fabiana S.
    Perez, Guillermo J.
    Selga, Elisabet
    STEM CELL RESEARCH, 2022, 60
  • [50] Generation of an induced pluripotent stem cell lines HMSCASTi001-A from peripheral blood mononuclear cells of a 35-year-old healthy male
    Zhang, Zhanchi
    Liu, Jingrui
    Zhang, Jiamin
    Xian, Xian
    Hao, Peipei
    Zhai, Yingtong
    Cheng, Jingke
    Yue, Fengming
    Luan, Feng
    Ma, Min
    Ren, Qian
    STEM CELL RESEARCH, 2022, 64
12345