Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female

被引：2
|
作者：
Nimonkar, Madhura Milind [1 ]
Arunachal, Gautham [2 ]
Dash, Suravi Sasmita [1 ]
Kandy, Swapna Kannothum [1 ]
Raghavendra, Kenchaiah [3 ]
Chetan, Ghati K. [2 ]
Mehta, Bhupesh [1 ]
Markandeya, Yogananda S. [1 ]
机构：
[1] Natl Inst Mental Hlth & Neurosci NIMHANS, Dept Biophys, Bengaluru, India
[2] Natl Inst Mental Hlth & Neuro Sci NIMHANS, Dept Human Genet, Bengaluru, Karnataka, India
[3] Natl Inst Mental Hlth & Neurosci NIMHANS, Dept Neurol, Bengaluru, India
来源：
STEM CELL RESEARCH | 2023年 / 70卷
关键词：
D O I：
10.1016/j.scr.2023.103130
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors-SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms.
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页数：4
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