B-cell cytokine responses to Porphyromonas gingivalis in patients with periodontitis and healthy controls

被引:4
|
作者
Danielsen, Anne Katrine [1 ,2 ]
Damgaard, Christian [1 ,2 ]
Massarenti, Laura [2 ]
ostrup, Peter [2 ,3 ]
Riis Hansen, Peter [4 ]
Holmstrup, Palle [1 ]
Nielsen, Claus H. [1 ,2 ]
机构
[1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Odontol, Sect Oral Biol & Immunopathol, Copenhagen, Denmark
[2] Copenhagen Univ Hosp, Inst Inflammat Res, Ctr Rheumatol & Spine Dis, Rigshosp, Copenhagen, Denmark
[3] Univ Copenhagen, Fac Hlth & Med Sci, Costerton Biofilm Ctr, Dept Immunol & Microbiol, Copenhagen, Denmark
[4] Univ Copenhagen, Herlev & Gentofte Hosp, Dept Cardiol, Hellerup, Denmark
关键词
B cells; cytokines; Porphyromonas gingivalis; periodontitis; PERI-IMPLANT DISEASES; KEY; CLASSIFICATION; AUTOIMMUNITY;
D O I
10.1002/JPER.22-0438
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
BackgroundCytokine-producing B cells play a well-established role in modifying immune responses in chronic inflammatory diseases. We characterized B-cell cytokine responses against periodontitis-associated bacteria in patients with periodontitis. MethodsBlood and saliva samples were collected from patients with periodontitis grade B (N = 31) or grade C (N = 25), and 25 healthy controls (HCs). Mononuclear cells were stimulated with Porphyromonas gingivalis, Fusobacterium nucleatum, Staphylococcus epidermidis, or Cutibacterium acnes, and B-cell production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, interferon (IFN)-gamma, IL-10 and transforming growth factor (TGF)-beta by B cells was assessed by flow cytometry. ResultsHCs had higher baseline frequencies of B cells producing IFN-gamma or TNF-alpha than grade B patients, but only B cells from grade B patients showed significant differentiation into IFN-gamma-, TNF-alpha-, TGF-beta-, or IL-10-producing cells after challenge with P. gingivalis and into IFN-gamma-, TGF-beta-, or IL-10-producing cells after challenge F. nucleatum. Notably, the baseline frequency of IL-10-producing B cells from grade C patients correlated inversely with clinical attachment loss (AL). The major proportion of the IFN-gamma- and TGF-beta-producing B cells were CD27(+) memory cells, while the IL-10-producing B cells were mainly CD27(-)CD5(-). ConclusionsB cells from grade B patients, particularly those harboring P. gingivalis, showed proinflammatory B-cell responses to P. gingivalis. Moreover, the baseline frequency of IL-10-producing B cells in the grade C group correlated inversely with AL, suggesting a diminished immunoregulatory capacity of IL-10-producing B cells in these patients.
引用
收藏
页码:997 / 1007
页数:11
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