Chemical and Biological Studies of Endophytes Isolated from Marchantia polymorpha

被引:7
|
作者
Stelmasiewicz, Mateusz [1 ]
Swiatek, Lukasz [2 ]
Ludwiczuk, Agnieszka [1 ]
机构
[1] Med Univ Lublin, Dept Pharmacognosy Med Plant Garden, PL-20093 Lublin, Poland
[2] Med Univ Lublin, Dept Virol, SARS Lab, PL-20093 Lublin, Poland
来源
MOLECULES | 2023年 / 28卷 / 05期
关键词
liverwort endophytes; diketopiperazines; cytotoxic and anticancer activity; antiviral activity; HIGH-SPEED; BRYOPHYTES; DIVERSITY; DIKETOPIPERAZINES; DERIVATIVES;
D O I
10.3390/molecules28052202
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Natural bioresources, predominantly plants, have always been regarded as the richest source of drugs for diseases threatening humanity. Additionally, microorganism-originating metabolites have been extensively explored as weapons against bacterial, fungal, and viral infections. However, the biological potential of metabolites produced by plant endophytes still remains understudied, despite significant efforts reflected in recently published papers. Thus, our goal was to evaluate the metabolites produced by endophytes isolated from Marchantia polymorpha and to study their biological properties, namely anticancer and antiviral potential. The cytotoxicity and anticancer potential were assessed using the microculture tetrazolium technique (MTT) against non-cancerous VERO cells and cancer cells-namely the HeLa, RKO, and FaDu cell lines. The antiviral potential was tested against the human herpesvirus type-1 replicating in VERO cells by observing the influence of the extract on the virus-infected cells and measuring the viral infectious titer and viral load. The most characteristic metabolites identified in the ethyl acetate extract and fractions obtained by use of centrifugal partition chromatography (CPC) were volatile cyclic dipeptides, cyclo(l-phenylalanyl-l-prolyl), cyclo(l-leucyl-l-prolyl), and their stereoisomers. In addition to the diketopiperazine derivatives, this liverwort endophyte also produced arylethylamides and fatty acids amides. The presence of N-phenethylacetamide and oleic acid amide was confirmed. The endophyte extract and isolated fractions showed a potential selective anticancer influence on all tested cancer cell lines. Moreover, the extract and the first separated fraction noticeably diminished the formation of the HHV-1-induced cytopathic effect and reduced the virus infectious titer by 0.61-1.16 log and the viral load by 0.93-1.03 log. Endophytic organisms produced metabolites with potential anticancer and antiviral activity; thus, future studies should aim to isolate pure compounds and evaluate their biological activities.
引用
收藏
页数:16
相关论文
共 50 条
  • [31] Whole-Genome Sequence of Paenibacillus marchantiae Isolated from the Liverwort Marchantia polymorpha subsp. ruderalis Ecotype BoGa
    Meierhenrich, Anja
    Frommer, Bianca
    Halpape, Wiebke
    Hildebrandt, Marvin
    Bleile, Isabell E.
    Helmig, Judith
    Zachgo, Sabine
    Braeutigam, Andrea
    Verwaaijen, Bart
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2023, 12 (07):
  • [32] Antifungal bis[bibenzyls] from the Chinese liverwort Marchantia polymorpha L.
    Niu, C
    Qu, JB
    Lou, HX
    CHEMISTRY & BIODIVERSITY, 2006, 3 (01) : 34 - 40
  • [33] Involvement of arabinogalactan proteins in protonemata development from cultured cells of Marchantia polymorpha
    Shibaya, T
    Kaneko, Y
    Sugawara, Y
    PHYSIOLOGIA PLANTARUM, 2005, 124 (04) : 504 - 514
  • [34] PHENANTHRENES AND OTHER PHENOLICS FROM IN-VITRO CULTURES OF MARCHANTIA-POLYMORPHA
    ADAM, KP
    BECKER, H
    PHYTOCHEMISTRY, 1994, 35 (01) : 139 - 143
  • [35] Chemical profiling of fungal endophytes of the genus Xylaria isolated from Coffea arabica
    Placeres, Angel L.
    Figueroa, Samuel O.
    Rodriguez, Ileana I.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 244
  • [36] Mangrove derived fungal endophytes - a chemical and biological perception
    Debbab, Abdessamad
    Aly, Amal H.
    Proksch, Peter
    FUNGAL DIVERSITY, 2013, 61 (01) : 1 - 27
  • [38] Mangrove derived fungal endophytes – a chemical and biological perception
    Abdessamad Debbab
    Amal H. Aly
    Peter Proksch
    Fungal Diversity, 2013, 61 : 1 - 27
  • [39] Isolation, identification, and biological characterization of bacterial endophytes isolated from Gunnera perpensa L
    Mahlangu, Siphiwe Godfrey
    Zulu, Nodumo
    Serepa-Dlamini, Mahloro Hope
    Tai, Siew Leng
    FEMS MICROBIOLOGY LETTERS, 2024, 371
  • [40] A comparative study of in vitro cytotoxicity and chemical constituents of wild and cultured plants of Marchantia polymorpha L.
    Mishra, Tripti
    Sahu, Vinay
    Meena, Sanjeev
    Pal, Mahesh
    Asthana, A. K.
    Datta, Dipak
    Upreti, D. K.
    SOUTH AFRICAN JOURNAL OF BOTANY, 2023, 157 : 274 - 278