Nitrogen transfer from root exudates to the rhizobiome: A 15N stem feeding method

被引：1
|
作者：
De Sena, Aidan [1 ]
Madramootoo, Chandra A. [1 ]
Whalen, Joann K. [2 ]
机构：
[1] McGill Univ, Dept Bioresource Engn, 21,111 Lakeshore Rd,Macdonald Campus, Sainte Anne De Bellevue, PQ H9X 3V9, Canada
[2] McGill Univ, Dept Nat Resource Sci, 21,111 Lakeshore Rd,Macdonald Campus, St Anne De Dellevue, PQ H9X 3V9, Canada
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2023年 / 186卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
15N isotope tracing; Cotton-wick method; Leaf tip feeding method; Rhizodeposition; Soil microbiome; Rhizosphere; RHIZODEPOSITION; TOXICITY; SOIL;
D O I：
10.1016/j.soilbio.2023.109159
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Plant roots exude nitrogen (N)-containing compounds, which are assimilated by the rhizobiome to maintain stoichiometric homeostasis. Various 15N-tracing methods exist to estimate the rhizobiome-N derived from root exudation but have never been validated with a full factorial experiment. We exposed ryegrass (Lolium multi-florum) to 15N solutions through different plant organs (stem or leaf feeding), with different 15N-tracers (urea or NH4NO3) and concentrations (64.5, 129 or 193 mmol 15N L-1). Stem feeding with either 15N-source at the highest concentration was the only effective way to quantify root exudate-N assimilation by the rhizobiome, and we recommend this 15N-labeling method for the reliable assessment of microbiome-N dynamics in the rhizosphere.
引用
收藏
页数：4
相关论文
共 50 条
  • [21] On the correlation between δ15N and nitrogen content in nanodiamonds from meteorites
    Fisenko, AV
    Verchovsky, AB
    Semjonova, LF
    METEORITICS & PLANETARY SCIENCE, 2005, 40 (09) : A48 - A48
  • [22] Herbicide application increases nitrogen (15N) exudation and root detachment of Brachiaria decumbens Stapf
    Damin, Virginia
    Trivelin, Paulo C. O.
    Carvalho, Saul J. P.
    Moraes, Milton F.
    Barbosa, Thiago G.
    PLANT AND SOIL, 2010, 334 (1-2) : 511 - 519
  • [23] Use of 15N stable isotope to quantify nitrogen transfer between mycorrhizal plants
    He, Xinhua
    Xu, Minggang
    Qiu, Guo Yu
    Zhou, Jianbin
    JOURNAL OF PLANT ECOLOGY, 2009, 2 (03) : 107 - 118
  • [24] Comparison of the difference method and 15N technique for studying the fate of nitrogen from plant residues in soil
    Breland, TA
    Hansen, S
    BIOLOGY AND FERTILITY OF SOILS, 1998, 26 (03) : 164 - 168
  • [25] A Comparison of Nitrogen Transfer and Transformation in Traditional Farming and the Rice-Duck Farming System by 15N Tracer Method
    Ebissa, Tchister Morrel
    Yang, Bo
    Guan, Yuanqing
    Tan, Bingchang
    Chen, Peizhen
    Wang, Lili
    Magwanga, Richard Odongo
    Zheng, Xiangqun
    AGRONOMY-BASEL, 2018, 8 (12):
  • [26] Comparison of the difference method and 15N technique for studying the fate of nitrogen from plant residues in soil
    T. A. Breland
    S. Hansen
    Biology and Fertility of Soils, 1998, 26 : 164 - 168
  • [27] Examining the fate of WWTP effluent nitrogen using δ15N–NH4+, δ15N–NO3− and δ15N of submersed macrophytes
    Jennifer L. A. Hood
    William D. Taylor
    Sherry L. Schiff
    Aquatic Sciences, 2014, 76 : 243 - 258
  • [28] Root exudates: a pathway for short-term N transfer from clover and ryegrass
    Paynel, F
    Murray, PJ
    Cliquet, JB
    PLANT AND SOIL, 2001, 229 (02) : 235 - 243
  • [29] Root exudates: a pathway for short-term N transfer from clover and ryegrass
    Florence Paynel
    Philip J Murray
    Jean Bernard Cliquet
    Plant and Soil, 2001, 229 : 235 - 243
  • [30] The origin of nitrogen on Jupiter and Saturn from the 15N/14N ratio
    Fletcher, Leigh N.
    Greathouse, T. K.
    Orton, G. S.
    Irwin, P. G. J.
    Mousis, O.
    Sinclair, J. A.
    Giles, R. S.
    ICARUS, 2014, 238 : 170 - 190
12345