1H-NMR protocol for rapid diagnosis of purine and pyrimidine metabolic disorders in urine

被引：1

作者：

Davoren, Elmarie ^{[1
]}

Mason, Shayne ^{[2
]}

机构：

[1] North West Univ, Ctr Human Metabol, ZA-2531 Potchefstroom, South Africa

[2] North West Univ, Biochem Dept, ZA-2531 Potchefstroom, South Africa

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 02期

关键词：

Metabolism; Metabolomics; NMR;

D O I：

10.1016/j.xpro.2023.102181

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Purine and pyrimidine disorders are often difficult to diagnose. Here, we present a 1H-NMR analysis protocol for the quantification of purines and pyrimidines in urine to diagnose associated disorders. We describe steps for pH adjustment, sample preparation, and 1H-NMR analysis and data analysis. The use of 1H-NMR requires a relatively small sample volume (1 mL) and minimal sample preparation. Analysis time produces accurate and reproducible data within 2 h.

引用

页数：12

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