A holistic approach for process intensification of nicotinamide mononucleotide production via high cell density cultivation under exponential feeding strategy

被引：6

作者：

Kafle, Saroj Raj ^{[1
]}

Kushwaha, Anamika ^{[1
]}

Goswami, Lalit ^{[1
]}

Maharjan, Anoth ^{[1
]}

Kim, Beom Soo ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Chem Engn, Cheongju 28644, Chungbuk, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2023年 / 390卷

基金：

新加坡国家研究基金会;

关键词：

Nicotinamide mononucleotide; High cell density; Exponential feeding strategy; Escherichia coli; RECOMBINANT ESCHERICHIA-COLI; FED-BATCH FERMENTATION; GLYCEROL; NAD(+);

D O I：

10.1016/j.biortech.2023.129911

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Nicotinamide mononucleotide (NMN) subsists in all living organisms and has drawn tremendous attention as a nutraceutical and pharmaceutical product for several diseases such as Alzheimer's, cancer, aging, and vascular dysfunction. Here, NMN was produced intracellularly in a high cell density bioreactor using an engineered Escherichia coli strain via exponential feeding of co-substrates. Fed-batch culture via exponential feeding of cosubstrate (glucose) and continuous feeding of substrate (nicotinamide) were performed using different cumulative nicotinamide concentrations. The highest concentration of 19.3 g/L NMN with a dry cell weight of 117 g/L was acquired from a cumulative nicotinamide concentration of 7.2 g/L with a conversion of 98 % from nicotinamide in 28 h. Further, liquid chromatography-mass spectrometry analysis validated the NMN production. This approach will be beneficial in achieving simultaneously low cost and ensuring high quality and quantity of NMN production.

引用

页数：9