Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker

被引：4
|
作者：
Kitao, Masahiro [1 ]
Hayashi, Ryuhei [1 ,2 ,3 ]
Nomi, Kimihito [4 ]
Kobayashi, Reiko [1 ]
Katayama, Tomohiko [1 ]
Takayanagi, Hiroshi [1 ,2 ]
Oguchi, Akiko [5 ]
Murakawa, Yasuhiro [5 ,6 ]
Nishida, Kohji [1 ,3 ,7 ]
机构：
[1] Osaka Univ, Grad Sch Med, Dept Ophthalmol, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Med, Dept Stem Cells & Appl Med, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Inst Open & Transdisciplinary Res Initiat, Integrated Frontier Res Med Sci Div, Suita, Osaka 5650871, Japan
[4] ROHTO Pharmaceut, Business Strategy Off, Osaka, Osaka 5440012, Japan
[5] RIKEN Ctr Integrat Med Sci, RIKEN IFOM Joint Lab Canc Genom, Yokohama, Kanagawa 2300045, Japan
[6] Kyoto Univ, Inst Adv Study Human Biol ASHBi, Kyoto, Kyoto 6068501, Japan
[7] Osaka Univ, Premium Res Inst Human Metaverse Med WPI PRIMe, Suita, Osaka 5650871, Japan
来源：
ISCIENCE | 2023年 / 26卷 / 07期
基金：
日本学术振兴会; 日本科学技术振兴机构;
关键词：
GOBLET CELLS; EXPRESSION; PROTEIN;
D O I：
10.1016/j.isci.2023.107016
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The conjunctival epithelium consists of conjunctival epithelial cells and goblet cells derived from conjunctival epithelial stem/progenitor cells. However, the source of these cells is not well known because no specific markers for conjunctival epithelial stem/progenitor cells have been discovered. Therefore, to identify conjunctival epithelial stem/progenitor cell markers, we performed single-cell RNA sequencing of a conjunctival epithelial cell population derived from human-induced pluripotent stem cells (hiPSCs). The following conjunctival epithelial markers were identified: BST2, SLC2A3, AGR2, TMEM54, OLR1, and TRIM29. Notably, BST2 was strongly positive in the basal conjunctival epithelium, which is thought to be rich in stem/progenitor cells. Moreover, BST2 was able to sort conjunctival epithelial stem/progenitor cells from hiPSC-derived ocular surface epithelial cell populations. BST2-positive cells were highly proliferative and capable of successfully generating conjunctival epithelial sheets containing goblet cells. In conclusion, BST2 has been identified as a specific marker of conjunctival epithelial stem/progenitor cells.
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页数：19
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