Tissue Extract from Brittle Star Undergoing Arm Regeneration Promotes Wound Healing in Rat

被引：3

作者：

Afshar, Alireza ^{[1
,2
,3
]}

Khoradmehr, Arezoo ^{[3
]}

Nowzari, Fariborz ^{[4
]}

Baghban, Neda ^{[3
]}

Zare, Masoud ^{[3
]}

Najafi, Maryam ^{[4
]}

Keshavarzi, Seyedeh Zahra ^{[4
]}

Zendehboudi, Fatemeh ^{[2
,3
]}

Mohebbi, Gholamhossein ^{[3
]}

Barmak, Alireza ^{[5
]}

Mohajer, Fatemeh ^{[2
,3
]}

Basouli, Nahid ^{[2
,3
]}

Keshtkar, Mohammadreza ^{[2
,3
]}

Iraji, Aida ^{[6
,7
]}

Aslani, Fatemeh Sari ^{[8
]}

Irajie, Cambyz ^{[9
]}

Nabipour, Iraj ^{[3
]}

Mahmudpour, Mehdi ^{[10
]}

Tanideh, Nader ^{[4
,11
]}

Tamadon, Amin ^{[1
,12
]}

机构：

[1] PerciaVista R&D Co, Shiraz 73, Iran

[2] Bushehr Univ Med Sci, Student Res Comm, Bushehr 75, Iran

[3] Bushehr Univ Med Sci, Persian Gulf Biomed Sci Res Inst, Persian Gulf Marine Biotechnol Res Ctr, Bushehr 75, Iran

[4] Shiraz Univ Med Sci, Stem Cells Technol Res Ctr, Shiraz 73, Iran

[5] Bushehr Univ Med Sci, Food Lab, Bushehr 75, Iran

[6] Shiraz Univ Med Sci, Med & Nat Prod Chem Res Ctr, Shiraz 73, Iran

[7] Shiraz Univ Med Sci, Cent Res Lab, Shiraz 73, Iran

[8] Shiraz Univ Med Sci, Mol Dermatol Res Ctr, Sch Med, Shiraz 73, Iran

[9] Shiraz Univ Med Sci, Sch Adv Med Sci & Technol, Dept Med Biotechnol, Shiraz 73, Iran

[10] Bushehr Univ Med Sci, Persian Gulf Biomed Sci Res Inst, Persian Gulf Trop Med Res Ctr, Bushehr 75, Iran

[11] Shiraz Univ Med Sci, Med Sch, Dept Pharmacol, Shiraz 73, Iran

[12] West Kazakhstan Marat Ospanov Med Univ, Dept Sci Work, Aktobe 030012, Kazakhstan

来源：

MARINE DRUGS | 2023年 / 21卷 / 07期

关键词：

wound healing; regeneration; proliferation; brittle star; rat; OPHIOCOMA-ERINACEUS; ANTIBACTERIAL ACTIVITY; ACID; OPHIUROIDEA; DERIVATIVES; GROWTH; PROLIFERATION; ANTIOXIDANT; ANTICANCER; INHIBITOR;

D O I：

10.3390/md21070381

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

This study set out to evaluate the wound healing properties of brittle star extracts in vitro and in vivo. Due to the great arm regeneration potential of the brittle star, Ophiocoma cynthiae, the present study aimed to evaluate the wound healing effect of hydroalcoholic extracts of brittle star undergoing arm regeneration in wound healing models. The brittle star samples were collected from Nayband Bay, Bushehr, Iran. After wound induction in the arm of brittle stars, hydroalcoholic extracts relating to different times of arm regeneration were prepared. The GC-MS analysis, in vitro MTT cell viability and cell migration, Western blot, and computational analysis tests were performed. Based on the in vitro findings, two BSEs were chosen for in vivo testing. Macroscopic, histopathological and biochemical evaluations were performed after treatments. The results showed positive proliferative effects of BSEs. Specifically, forty-two compounds were detected in all groups of BSEs using GC-MS analysis, and their biological activities were assessed. The MTT assay showed that the 14 d BSE had a higher proliferative effect on HFF cells than 7 d BSE. The cell migration assay showed that the wound area in 7 d and 14 d BSEs was significantly lower than in the control group. Western blot analysis demonstrated an increase in the expression of proliferation-related proteins. Upon the computational analysis, a strong affinity of some compounds with proteins was observed. The in vivo analysis showed that the evaluation of wound changes and the percentage of wound healing in cell migration assay in the 7 d BSE group was better than in the other groups. Histopathological scores of the 7 d BSE and 14 d BSE groups were significantly higher than in the other groups. In conclusion, the hydroalcoholic extract of O. cynthiae undergoing arm regeneration after 7 and 14 days promoted the wound healing process in the cell and rat skin wound healing model due to their proliferative and migratory biological activity.

引用

页数：31

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