Modelling cowpea beans α-galactosidase inactivation dependence on temperature and moisture content

被引:3
|
作者
Siguemoto, Erica [1 ]
Atmani, Lamiae [1 ]
Mestres, Christian [1 ]
Meot, Jean-Michel [1 ]
机构
[1] Univ Reunion, Univ Montpellier, Avignon Univ, Inst Agro,QualiSud,CIRAD,IRD, Montpellier, France
关键词
Oligosaccharides; Enzyme kinetics; Hydrothermal process; Pulses;
D O I
10.1016/j.lwt.2023.115571
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Hydrothermal treatment improves the digestibility of pulses through the coupled phenomena of enzymatic degradation and diffusion of galacto-oligosaccharides, despite the thermal inactivation of alpha-galactosidase at mild temperatures. To better understand the key process parameters that influence the balance between enzyme activity and its inactivation, the kinetics of alpha-galactosidase inactivation were investigated in a cowpea-water system at a broad range of temperatures (45 degrees C <= T <= 60 degrees C), moisture contents (0.16 g/g dry basis, d.b. <= W <= 0.85 g/g d.b.) and treatment times (5 min <= t <= 7.5 h). For moisture contents ranging from 0.25 to 0.85 g/g d.b., the higher the temperature the faster the inactivation of alpha-galactosidase; while for 0.16 g/g d.b., the relative enzymatic inactivation was not temperature-dependent. A first-order model was successfully fitted to the experimental data, whereas the temperature dependence of inactivation rate followed the Arrhenius model. The influence of moisture content on the enzymatic inactivation rate was remarkable. The inactivation rate constant at excess water (0.85 g/g d.b.) was five times higher than the samples at limited water (0.35 g/g d.b.) condition. The results showed moisture-dependent alpha-galactosidase inactivation kinetics mainly at mild temperatures (55 and 60 degrees C), with implications for the representation of diffusion-reaction phenomena during hydrothermal processing of pulses.
引用
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页数:5
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