A GFP Inspired 8-Methoxyquinoline-Derived Fluorescent Molecular Sensor for the Detection of Zn2+ by Two-Photon Microscopy

被引：2

作者：

Csomos, Attila ^{[1
,2
]}

Madarasz, Miklos ^{[3
]}

Turczel, Gabor ^{[4
]}

Cseri, Levente ^{[3
,5
]}

Bodor, Andrea ^{[6
]}

Matuscsak, Anett ^{[7
]}

Katona, Gergely ^{[8
]}

Kovacs, Ervin ^{[8
,9
]}

Rozsa, Balazs ^{[3
,7
,8
]}

Mucsi, Zoltan ^{[1
,3
,10
]}

机构：

[1] Femtonics Ltd, Tuzolto Utca 59, H-1094 Budapest, Hungary

[2] Eotvos Lorand Univ, Hevesy Gyorgy PhD Sch Chem, Pazmany Peter Setany 1-A, H-1117 Budapest, Hungary

[3] BrainVisionCenter, Liliom Utca 43-45, H-1094 Budapest, Hungary

[4] HUN REN Res Ctr Nat Sci, NMR Res Lab, Magyar Tudosok Korutja 2, H-1117 Budapest, Hungary

[5] Budapest Univ Technol & Econ, Dept Organ Chem & Technol, Muegyetem Rakpart 3, H-1111 Budapest, Hungary

[6] Eotvos Lorand Univ, Inst Chem, Analyt & BioNMR Lab, Pazmany Peter Setany 1-A, H-1117 Budapest, Hungary

[7] HUN REN Inst Expt Med, Lab Funct Network & Dendrit Imaging 3D, Szigony Utca 43, H-1083 Budapest, Hungary

[8] Pazmany Peter Catholic Univ, Photon Measurement Technol Res Grp 2, Prater Utca 50-A, H-1083 Budapest, Hungary

[9] HUN REN Res Ctr Nat Sci, Polymer Chem & Phys Res Grp, Magyar Tudosok Korutja 2, H-1117 Budapest, Hungary

[10] Univ Miskolc, Fac Mat & Chem Sci, H-3515 Miskolc, Hungary

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2024年 / 30卷 / 31期

关键词：

zinc-ion; two-photon; chemosensor; fluorescence; 8-methoxyquinoline; ZINC IONS; PROBE; ZN(II); TISSUE; CELLS;

D O I：

10.1002/chem.202400009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An effective, GFP-inspired fluorescent Zn2+ sensor is developed for two-photon microscopy and related biological application that features an 8-methoxyquinoline moiety. Excellent photophysical characteristics including a 37-fold fluorescence enhancement with excitation and emission maxima at 440 nm and 505 nm, respectively, as well as a high two-photon cross-section of 73 GM at 880 nm are reported. Based on the experimental data, the relationship between the structure and properties was elucidated and explained backed up by DFT calculations, particularly the observed PeT phenomenon for the turn-on process. Biological validation and detailed experimental and theoretical characterization of the free and the zinc-bound compounds are presented.

引用

页数：6

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