Protocol Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models

被引：0

作者：

Chantrel, Jeremy ^{[1
,2
]}

Chen, Cheng ^{[1
]}

Zhang, Jun ^{[3
]}

Li, Han ^{[1
]}

机构：

[1] Univ Paris Cite, Inst Pasteur, Dept Dev & Stem Cell Biol, Cellular Plast Age Related Pathol,CNRS UMR 3738, 25 Rue Dr Roux, F-75015 Paris, France

[2] Sorbonne Univ, Coll Doctoral, F-75005 Paris, France

[3] Telecom Paris, 19 Pl Marguer Perey, F-91120 Palaiseau, France

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cell Biology; Cell culture; Microscopy; Model Organisms; Stem Cells;

D O I：

10.1016/j.xpro.2023.102106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a protocol to evaluate the impact of senescence secretome on reprog-ramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in vivo reprogram-ming, we detail the steps to identify senescent and reprogrammed cells in mouse skeletal muscle, followed by semi-automatic quantification. This protocol can be used to study the effect of paracrine senescence on cellular plasticity. For complete details on the use and execution of this protocol, please refer to von Joest et al. (2022).1

引用

页数：20