Protocol Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models

被引：0

作者：

Chantrel, Jeremy ^{[1
,2
]}

Chen, Cheng ^{[1
]}

Zhang, Jun ^{[3
]}

Li, Han ^{[1
]}

机构：

[1] Univ Paris Cite, Inst Pasteur, Dept Dev & Stem Cell Biol, Cellular Plast Age Related Pathol,CNRS UMR 3738, 25 Rue Dr Roux, F-75015 Paris, France

[2] Sorbonne Univ, Coll Doctoral, F-75005 Paris, France

[3] Telecom Paris, 19 Pl Marguer Perey, F-91120 Palaiseau, France

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cell Biology; Cell culture; Microscopy; Model Organisms; Stem Cells;

D O I：

10.1016/j.xpro.2023.102106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a protocol to evaluate the impact of senescence secretome on reprog-ramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in vivo reprogram-ming, we detail the steps to identify senescent and reprogrammed cells in mouse skeletal muscle, followed by semi-automatic quantification. This protocol can be used to study the effect of paracrine senescence on cellular plasticity. For complete details on the use and execution of this protocol, please refer to von Joest et al. (2022).1

引用

页数：20

共 21 条

[1] Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to 13-like Cells
Elhanani, Ofer
Walker, Michael D.
STAR PROTOCOLS, 2020, 1 (02):
[2] Protocol for remapping of place cells in disease mouse models
Jun, Heechul
Chavez, Jasmine
Bramian, Allen
Igarashi, Kei M.
STAR PROTOCOLS, 2021, 2 (03):
[3] Protocol to generate traceable CAR T cells for syngeneic mouse cancer models
Zhang, Duo
Krimitza, Elisavet
Han, Katherine
Su, Ruiying
Xu, David J.
Xu, Jaiden R.
Gong, Yanqing
Fan, Yi
STAR PROTOCOLS, 2024, 5 (01):
[4] From cells to ageing: a review of models and mechanisms of cellular senescence and their impact on human ageing
de Magalhaes, JP
EXPERIMENTAL CELL RESEARCH, 2004, 300 (01) : 1 - 10
[5] Protocol to induce cell labeling in vivo and to trace labeled hematopoietic cells using mouse models
Syed, Alina
Kobayashi, Michihiro
Yoshimoto, Momoko
STAR PROTOCOLS, 2024, 5 (04):
[6] Optimized protocol for quantification of mitochondrial non-heme and heme iron content in mouse tissues and cultured cells
Sato, Tatsuya
Chang, Hsiang-Chun
Sawicki, Konrad T.
Ardehali, Hossein
STAR PROTOCOLS, 2023, 4 (01):
[7] Impact of topographical analysis on quantitative microscopy in cultured cells: Application to hormone therapy evaluation
Wallet, F
Berthois, Y
Martin, PM
Dussert, C
Palmari, J
BULLETIN DU CANCER, 1997, 84 (06) : 589 - 595
[8] Protocol for the isolation of mouse senescence- associated CD4+ T cells using flow cytometry and functional assays
Fukushima, Yuji
Minato, Nagahiro
Hattori, Masakazu
STAR PROTOCOLS, 2023, 4 (03):
[9] An updated Barnes maze protocol for assessing the outcome of controlled cortical impact mouse models of traumatic brain injury
Gee, Connor C.
Steffen, Rylie
Kievit, Forrest M.
JOURNAL OF NEUROSCIENCE METHODS, 2023, 392
[10] Protocol for a semi-quantitative approach to identify protein S-palmitoylation in cultured cells by acyl biotin exchange assay
Leishman, Stuart
Aljadeed, Najd M.
Anand, Paras K.
STAR PROTOCOLS, 2024, 5 (02):

← 1 2 3 →