Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

被引:5
|
作者
Wu, Shuo [1 ]
Zhang, Lijie [2 ]
Zhang, Ruidan [3 ]
Yang, Kang [4 ]
Wei, Qin [5 ]
Jia, Qiyu [1 ]
Guo, Jian [1 ]
Ma, Chuang [1 ]
机构
[1] Xinjiang Med Univ, Dept Microrepair & Reconstruct, Affiliated Hosp 1, Urumqi, Peoples R China
[2] Xinjiang Med Univ, Dept Neurol, Affiliated Hosp 2, Urumqi, Peoples R China
[3] Guangdong New Omega Med Ctr, Guangzhou, Peoples R China
[4] Hand & Foot Microsurg Third Peoples Hosp Xinjiang, Urumqi, Peoples R China
[5] Xinjiang Med Univ, Anim Expt Ctr, Urumqi, Peoples R China
基金
中国国家自然科学基金;
关键词
BMSCs; distraction osteogenesis (DO); PDGF-BB; rat; osteogenesis; femur; IN-VIVO; THERAPY;
D O I
10.3389/fbioe.2023.1110703
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: In the clinical treatment of large bone defects, distraction osteogenesis can be used. However, some patients may suffer from poor bone regeneration, or even delayed healing or non-union. Problems with the aggregation and proliferation of primary osteoblasts, or problems with the differentiation of primary osteoblasts will lead to poor bone regeneration. Therefore, supplementing exogenous primary osteoblasts and growth factors when using distraction osteogenesis may be a treatment plan with great potential.Methods: Bone marrow mesenchymal stem cells (BMSCs) were extracted from rats and cultured. Subsequently, Recombinant Rat Platelet-derived Growth Factor BB (rrPDGF-BB) was used to induce bone marrow mesenchymal stem cells. At the same time, male adult rats were selected to make the right femoral distraction osteogenesis model. During the mineralization period, phosphate buffer salt solution (control group), non-induction bone marrow mesenchymal stem cells (group 1) and recombinant rat platelet-derived growth factor BB intervened bone marrow mesenchymal stem cells (group 2) were injected into the distraction areas of each group. Then, the experimental results were evaluated with imaging and histology. Statistical analysis of the data showed that the difference was statistically significant if p < 0.05.Results: After intervention with recombinant rat platelet-derived growth factor BB on bone marrow mesenchymal stem cells, the cell morphology changed into a thin strip. After the cells were injected in the mineralization period, the samples showed that the callus in group 2 had greater hardness and the color close to the normal bone tissue; X-ray examination showed that there were more new callus in the distraction space of group 2; Micro-CT examination showed that there were more new bone tissues in group 2; Micro-CT data at week eight showed that the tissue volume, bone volume, percent bone volume, bone trabecular thickness, bone trabecular number and bone mineral density in group 2 were the largest, and the bone trabecular separation in group 2 was the smallest. There was a statistical difference between the groups (p < 0.05); HE staining confirmed that group 2 formed more blood vessels and chondrocytes earlier than the control group. At 8 weeks, the bone marrow cavity of group 2 was obvious, and some of them had been fused.Conclusion: The study confirmed that injecting bone marrow mesenchymal stem cellsBB into the distraction space of rats can promote the formation of new bone in the distraction area and promote the healing of distraction osteogenesis.
引用
收藏
页数:13
相关论文
共 50 条
  • [31] Osteogenesis of bone marrow mesenchymal stem cell in hyperglycemia
    Luo, Meng
    Zhao, Zhihe
    Yi, Jianru
    FRONTIERS IN ENDOCRINOLOGY, 2023, 14
  • [32] Ultrastructural analysis of human bone marrow mesenchymal stem cells during in vitro osteogenesis and chondrogenesis
    Teti, Gabriella
    Cavallo, Carola
    Grigolo, Brunella
    Giannini, Sandro
    Facchini, Andrea
    Mazzotti, Antonio
    Falconi, Mirella
    MICROSCOPY RESEARCH AND TECHNIQUE, 2012, 75 (05) : 596 - 604
  • [33] Changes in blood supply and bone regeneration induced by nicotine during distraction osteogenesis
    Ma, Li
    Zheng, Li Wu
    Cheung, Lim Kwong
    BONE, 2008, 42 : S60 - S60
  • [34] Toward the identification of mesenchymal stem cells in bone marrow and peripheral blood for bone regeneration
    Smiler, Dennis
    Soltan, Muna
    Albitar, Maher
    IMPLANT DENTISTRY, 2008, 17 (03) : 236 - 244
  • [35] Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects: In vivo study
    Al-Qadhi, Gamilah
    Soliman, Malak
    Abou-Shady, Iman
    Rashed, Laila
    TISSUE & CELL, 2020, 63
  • [36] HAIR FOLLICLE STEM CELLS AND BONE MARROW MESENCHYMAL STEM CELLS IN BLADDER REGENERATION
    Joachimiak, R.
    Bajek, A.
    Cieslak, Z.
    Drewa, T.
    Gagat, M.
    Grzanka, A.
    Marszalek, A.
    Debski, R.
    EUROPEAN UROLOGY SUPPLEMENTS, 2010, 9 (06) : 632 - 633
  • [37] HAIR FOLLICLE STEM CELLS AND BONE MARROW MESENCHYMAL STEM CELLS IN BLADDER REGENERATION
    Joachimiak, R.
    Bajek, A.
    Drewa, T.
    Cieslak, Z.
    Gagat, M.
    Grzanka, A.
    Bodnar, M.
    Marszalek, A.
    Debski, R.
    EUROPEAN UROLOGY SUPPLEMENTS, 2011, 10 (02) : 214 - 214
  • [38] Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis
    Sun, Quan
    Nakata, Hidemi
    Yamamoto, Maiko
    Kasugai, Shohei
    Kuroda, Shinji
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (11) : 7592 - 7601
  • [39] Bone Regeneration with Rabbit Bone Marrow-Derived Mesenchymal Stem Cells and Bone Graft Materials
    Lee, Ji-Eun
    Heo, Seong-Joo
    Koak, Jai-Young
    Kim, Seong-Kyun
    Han, Chong-Hyun
    INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, 2012, 27 (06) : 1389 - 1399
  • [40] Kartogenin Improves Osteogenesis of Bone Marrow Mesenchymal Stem Cells via Autophagy
    Yan, Huichun
    Yu, Tingting
    Li, Jing
    Zhang, Ting
    Li, Qian
    Zhou, Yanheng
    Liu, Dawei
    STEM CELLS INTERNATIONAL, 2022, 2022