The study investigates the impact of incorporating multi-scale nano-zinc oxide (nano-ZnO) particles into a PG 64-22 asphalt binder, exploring changes in morphology, rheology, and physical properties. Utilizing nano-ZnO particles of varying sizes (10-30 nm, 50-70 nm, 100-200 nm) and concentrations (1-5%), the research employs Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) for morphological analysis and Dynamic Shear Rheometer (DSR) testing for rheological properties. The findings reveal substantial improvements in binder elasticity and rutting resistance, with notable enhancements in physical attributes such as lower penetration grades, higher ductility, and increased softening points. Additionally, nano-ZnO modified binders demonstrate superior aging resistance, maintaining their enhanced properties after both short-term and longterm aging tests. This comprehensive study underscores the potential of nano-ZnO as an effective modifier in asphalt binders, promising increased durability and resilience against common roadway distresses.