CgTNF-2 promotes the proliferation of haemocytes by regulating the expressions of CgRunx and cell cycle related genes in the Pacific oyster Crassostrea gigas

被引:5
|
作者
Wu, Wei [1 ,2 ,4 ]
Sun, Jiejie [2 ,4 ]
Dong, Miren [2 ,4 ]
Yang, Qian [2 ,4 ]
Yang, Wenwen [2 ,4 ]
Zhang, Tong [2 ,4 ]
Wang, Lingling [2 ,3 ,4 ,5 ]
Song, Linsheng [2 ,3 ,4 ,5 ]
机构
[1] Liaoning Normal Univ, Sch Life Sci, Dalian 116029, Peoples R China
[2] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Control, Dalian 116023, Peoples R China
[3] Southern Lab Ocean Sci & Engn, Zhuhai 519000, Guangdong, Peoples R China
[4] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
[5] Dalian Ocean Univ, Dalian Key Lab Prevent & Control Aquat Anim Dis, Dalian 116023, Peoples R China
基金
美国国家科学基金会;
关键词
Crassostrea gigas; Tumor necrosis factor; Granulocytes; Haemocyte proliferation; TUMOR-NECROSIS-FACTOR; FACTOR-ALPHA; TNF-ALPHA; MOLECULAR-CLONING; SUPERFAMILY; HOMOLOG; ACTIVATION; DEPENDENCE; LOZENGE; BLOOD;
D O I
10.1016/j.fsi.2022.108478
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A TNF-alpha family member, CgTNF-2, was previously identified from the oyster Crassostrea gigas to involve in the antibacterial response. In the present study, the role of CgTNF-2 in mediating the proliferation of haemocytes was further explored. The mRNA expression of CgTNF-2 in granulocytes was significantly higher than that in semigranulocytes and agranulocytes, and the percentages of CgTNF-2 antibody labeled cells in agranulocytes, semi-granulocytes and granulocytes were 19.15%, 40.25% and 94.07%, respectively. After the treatment with rCgTNF-2, the percentage of EdU+ cells in haemocytes increased significantly (1.77-fold, p < 0.05) at 6 h compared with that in rGST-treated group, and the mRNA expressions of CgRunx, CgCyclin A, CgCDK2 and CgCDC45 in haemocytes all increased significantly (p < 0.05), which were 1.94-fold, 2.13-fold, 1.97-fold, 1.76fold of that in rGST-treated group, respectively. Meanwhile, the protein abundance of CgRunx and CgCyclin A in the haemocytes of oysters in the rCgTNF-2-treated group increased, and the percentage of PI+ haemocytes in S phase also increased significantly (2.19-fold, p < 0.05) compared with that in rGST-treated group. These results collectively confirmed that CgTNF-2 was highly expressed in granulocytes and involved in the proliferation of haemocytes by regulating the expressions of CgRunx and cell cycle related genes in C. gigas.
引用
收藏
页数:9
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