Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus

被引：0
|
作者：
Chocholova, Eva [1 ]
Roudnicky, Pavel [2 ]
Potesil, David [2 ]
Fialova, Dana [1 ]
Krystofova, Karolina [2 ,3 ]
Drozdova, Eva [1 ]
Zdrahal, Zbynek [2 ,4 ]
机构：
[1] Masaryk Univ, Fac Sci, Dept Expt Biol, Lab Biol & Mol Anthropol, Brno 62500, Czech Republic
[2] Masaryk Univ, Cent European Inst Technol, Mendel Ctr Plant Genom & Prote, Brno 62500, Czech Republic
[3] Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, Brno 62500, Czech Republic
[4] Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, Brno 62500, Czech Republic
来源：
JOURNAL OF PROTEOME RESEARCH | 2023年 / 22卷 / 10期
关键词：
bioarcheology; paleoproteomics; ancient DNA; ancient proteins; dental calculus; GLUTAMINE DEAMIDATION; SUPRAGINGIVAL; DEGRADATION; PEPTIDES; DIETARY;
D O I：
10.1021/acs.jproteome.3c00370
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
引用
收藏
页码：3311 / 3319
页数：9
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