Fractionation and characterization of cell wall polysaccharides from the brown alga Cladosiphon okamuranus

被引:5
|
作者
Awanthi, Mahanama Geegana Gamage [1 ]
Umosa, Manatsu [2 ]
Yuguchi, Yoshiaki [3 ]
Oku, Hirosuke [1 ,4 ]
Kitahara, Kanefumi [1 ,5 ]
Ito, Michihiro [4 ]
Tanaka, Atsuko [6 ]
Konishi, Teruko [1 ,2 ]
机构
[1] Kagoshima Univ, United Grad Sch Agr Sci, 1-21-24 Korimoto, Kagoshima 8900065, Japan
[2] Univ Ryukyus, Dept Biosci & Biotechnol, Fac Agr, 1 Senbaru, Nishihara, Okinawa 9030213, Japan
[3] Osaka Electrocommun Univ, Fac Engn, 18-8 Hatsucho, Neyagawa, Osaka 5728530, Japan
[4] Univ Ryukyus, Ctr Mol Biosci, Trop Biosphere Res Ctr, 1 Senbaru, Nishihara, Okinawa 9030213, Japan
[5] Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, 1-21-24 Korimoto, Kagoshima 8900065, Japan
[6] Univ Ryukyus, Dept Chem Biol & Marine Sci, Fac Sci, 1 Senbaru, Nishihara, Okinawa 9030213, Japan
基金
日本学术振兴会;
关键词
Brown algae; Cladosiphon okamuranus; Cell wall polysaccharide; Fractionation; SULFATED POLYSACCHARIDES; FUCUS-SERRATUS; FUCOIDAN;
D O I
10.1016/j.carres.2022.108722
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brown algae contain a polysaccharide-rich cell wall, mainly composed of alginate and fucoidan which have been extensively studied for their individual structure and bioactivities. Particularly, the cell wall of Cladosiphon okamuranus is rich in fucoidan rather than alginate. However, little is known about its arrangement or interlinking with other polysaccharides such as cellulose in the cell wall. To determine its structure in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC-I), HC-II, and cellulose. Almost 80% of the total cell wall recovered from alcohol insoluble residue in C. okamuranus consisted of HW and HC-I, which mainly contained fucoidan composed of fucose, glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.9 and 1.0:0.2:0.3, respectively. Methylation analysis revealed that fucoidan in HW and HC-I structurally differed in terms of content of sulfate, and sugar residue which was 1,4linked xylose and 1,4-linked fucose. Small angle X-ray scattering measurements also showed distinct conformational differences between HW and HC-I. These structural heterogeneities of fucoidan may be related to their localization, and fucoidan in HC-I may be involved in reinforcing cell wall structure by cross-linking to cellulose.
引用
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页数:7
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