Vaginal Microbiota Profile in first-trimester miscarriages cases

Due to its rising prevalence, first-trimester miscarriage is getting more attention. Abortion etiology and pathology, especially in non-pathological cases, are unknown. In this study, 435 vaginal swabs were collected from terized the vaginal microbiota diversity and composition in first-trimester abortion and investigated the association between bacterial vaginosis and abortion before 12 weeks. Cultural, morphological, and biochemical characteristics for each bacterial genus were discovered, and VITEK-2 system was used to identify isolated bacteria. Samples from each type of bacteria were selected for sequencing utilizing 16 rRNA genes examining V4-V8 region for bacterial profiles. Bacterial vaginitis was found in 412 (94.7%) first-trimester miscarriages. Six Gram-positive and four Gram-negative bacteria were found in these 412 samples. Microorganism distribution varied Gram-positive bacteria Staphylococcus aureus (86) 20.87%, Enterococcus faecalis (31) 7.52%, and Staphylococcus haemolyticus (12) 2.91%. Gram-negative bacteria including E. coli (107) 25.97%, Klebsiella pneumonia (76) 18.45%, Pseudomonas aeruginosa (29) 7.04% and Proteus mirabilis (12) 2.91%. Staphylococcus aureus had the highest rate of isolation at (86) 20.87%, while Lactobacillus equicursoris had the lowest rate at (14) 3.4%. Overall, the rate of isolation for Gram-negative bacteria (224) was 54.4%, while the rate for Gram-positive bacteria (188) was 45.6%.