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An Investigation of Equine Sperm Quality Following Cryopreservation at Low Sperm Concentration and Repeated Freeze-Thawing
被引:2
|作者:
Morse-Wolfe, Bethany
[1
,2
,5
]
Bleach, Emma
[3
]
Kershaw, Claire
[4
]
机构:
[1] Harper Adams Univ, Anim Sci Dept, Newport, Wales
[2] Stall AI Serv Ltd, Chapelfield Stud, Whitchurch, Shrops, England
[3] Harper Adams Univ, Agr & Environm Dept, Newport, Wales
[4] Harper Adams Univ, Anim Hlth Behav & Welf Dept, Newport, Wales
[5] Univ Ctr Reaseheath, Nantwich CW5 6DF, Cheshire, England
关键词:
Stallion;
Spermatozoa;
Cryopreservation;
Low concentration;
Refreeze;
PLASMA-MEMBRANE INTEGRITY;
MOTION CHARACTERISTICS;
HYSTEROSCOPIC INSEMINATION;
PREGNANCY RATES;
STALLION SEMEN;
LOW NUMBERS;
SPERMATOZOA;
DILUTION;
CRYOPROTECTANTS;
FERTILITY;
D O I:
10.1016/j.jevs.2022.104167
中图分类号:
S85 [动物医学(兽医学)];
学科分类号:
0906 ;
摘要:
Stallion spermatozoa are typically cryopreserved at 200 to 300 million sperm/ml; however recent ad-vances such as intracytoplasmic sperm injection (ICSI) requires only one spermatozoon, wasting many, after thawing a whole straw. Cryopreserving at concentrations less than the current standard or refreez-ing thawed spermatozoa could maximize the use of genetically valuable animals and reduce waste. This investigation aimed to identify if lowering the sperm concentration for cryopreservation affected post -thaw quality after one and two freeze-thaw cycles. Nine ejaculates were collected from three fertile, "good freezer" stallions (post-thaw motility >35%) for experiment 1. Each ejaculate was split into eight treatments: five, 10, 20, 50, 100, 200, 300, 400 million sperm/ml and cryopreserved. Post-thaw: motility, viability, acrosome integrity and oxidative stress were assessed. Experiment 2, straws from experiment 1 (300 million sperm/ml) were thawed, diluted to 20 million sperm/ml or left undiluted (control) and refrozen. Post-thaw motility and viability were assessed. In experiment 1 sperm concentration did not affect post-thaw total motility (TM), progressive motility (PM) or viability at 50 to 400 million sperm/ml ( P > .05). Whilst sperm concentrations of five to 20 million/ml did differ (post-thaw TM and PM). Both refreezing and reducing spermatozoa concentration, decreased TM, PM and viability ( P < .05) after two freeze-thaw cycles. These results suggest cryopreserving at sperm concentrations as low as 50 million/ml maintains spermatozoa quality in good freezer stallions. Spermatozoa maintained some motility and via-bility when initially cryopreserved at 20 million sperm/ml and after two freeze-thaw cycles but research should investigate more optimal conditions.(c) 2022 Elsevier Inc. All rights reserved.
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