Life, the genome and everything

被引：0
|
作者：
Rosenberg, Susan M. [1 ,2 ,3 ,4 ]
机构：
[1] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Biochem & Mol Biol, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[4] Baylor Coll Med, Dan Duncan Comprehens Canc Ctr, Houston, TX 77030 USA
来源：
JOURNAL OF BACTERIOLOGY | 2023年 / 205卷 / 12期
基金：
美国国家卫生研究院;
关键词：
endogenous DNA damage; homology-directed DNA repair; chromosome segregation; Escherichia coli; genome integrity; DNA replication; SSB single-strand binding protein; RecG; mutations; evolution; STRESS-INDUCED MUTAGENESIS; RECOMBINATION; PROTEIN; REPLICATION; RECG;
D O I：
10.1128/jb.00272-23
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In this issue of the Journal of Bacteriology, N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023, https//doi.org/10.1128/jb.00184-23) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins' identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) "strand-exchange" intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing "death-by-recombination." DNA damage is managed to regulate crucial but potentially lethal HDR.
引用
收藏
页数：5
相关论文
共 50 条
12345