Imaged capillary isoelectric focusing employing fluorocarbon and methylcellulose coated fused silica capillary for characterization of charge heterogeneity of protein biopharmaceuticals

被引:6
|
作者
Kwok, Teresa [1 ]
Chan, She Lin [1 ]
Shi, Jessica [2 ]
Zhou, Mike [1 ]
Schaefer, Anna [1 ]
Bo, Tao [1 ]
Li, Victor [1 ]
Huang, Tiemin [1 ]
Chen, Tong [1 ]
机构
[1] Adv Electrophoresis Solut Ltd, Cambridge, ON, Canada
[2] McGill Univ, Fac Sci, Montreal, PQ, Canada
关键词
antibody; coated capillary; fluorocarbon; imaged capillary isoelectric focusing; mass spectrometry; MONOCLONAL-ANTIBODIES;
D O I
10.1002/sscp.202200160
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Identifying and characterizing charge heterogeneity is essential for the successful development and production of biopharmaceuticals. Imaged capillary isoelectric focusing technology based on isoelectric point differentiation has been becoming the gold standard of quality and manufactory process control in the biopharmaceutical industry due to its high-resolution characterization of protein charge variants with high throughput. Fluorocarbon-coated capillaries are widely used in the imaged capillary isoelectric focusing to suppress the electroosmotic flow and minimize the protein adsorption onto the fused silica capillary inner surface. Additionally, polymers such as methylcellulose are usually added to the sample solution to serve as a dynamic capillary coating that improves the peak shape and resolution during the imaged capillary isoelectric focusing separation, especially for complex proteins. However, the addition of methylcellulose tends to result in tedious operation, "spike peaks" from bubble generation, and frequent capillary blockage during the imaged capillary isoelectric focusing separation. In addition, methylcellulose is not compatible with mass spectrometry and easily produces the contamination of mass spectrometry ion source when carrying out imaged capillary isoelectric focusing-mass spectrometry direct coupling. Recently, a new imaged capillary isoelectric focusing method was developed employing a methylcellulose-coated capillary cartridge, to avoid the addition of methylcellulose which is then present throughout the whole analysis. When applied to protein drug characterization the established imaged capillary isoelectric focusing method demonstrated high repeatability, stable coatings, outstanding separation efficiency, and excellent isoelectric point differentiation. In addition, we compared imaged capillary isoelectric focusing separation using the methylcellulose-coated capillary with that utilizing the routinely coated capillary such as fluorocarbon, illustrating that methylcellulose coating provided consistent results and could be seamlessly integrated into an existing drug discovery process. Finally, the methylcellulose-coated fused silica capillary was applied to imaged capillary isoelectric focusing-mass spectrometry for characterizing protein charge variants allowing reliable identification of mass spectrometry after imaged capillary isoelectric focusing separation. This can greatly simplify the operation steps and prevent the contamination of mass spectrometry ion source that often results from using routinely coated capillaries ultimately making this an essential innovation of imaged capillary isoelectric focusing-mass spectrometry that greatly improves the imaged capillary isoelectric focusing compatibility with mass spectrometry.
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页数:9
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