Vitamins and minerals fortified emulsion of omega-3 fatty acids for the management of preterm birth: In-vitro, in-silico, and in-vivo studies

被引:6
|
作者
Thakur, Shubham [1 ]
Singh, Amrinder [1 ]
Kaur, Manjot [1 ]
Reza, Navid [1 ]
Kumar, Nitish [1 ]
Kour, Rasdeep [2 ]
Kaur, Satwinderjeet [3 ]
Bedi, Preet Mohinder Singh [1 ]
Jain, Subheet Kumar [1 ,3 ,4 ]
机构
[1] Guru Nanak Dev Univ, Dept Pharmaceut Sci, Amritsar 143005, India
[2] Guru Nanak Dev Univ, Dept Bot & Environm Sci, Amritsar 143005, India
[3] Guru Nanak Dev Univ, Ctr Basic & Translat Res Hlth Sci, Amritsar 143005, India
[4] Guru Nanak Dev Univ, Ctr Basic & Translat Res Hlth Sci, Dept Pharmaceut Sci, Amritsar 143005, India
关键词
Omega-3 fatty acids; Vitamins; Minerals; Fortified emulsions; In-silico screening; Preterm birth; BIRTH-WEIGHT; E-TPGS; RHEOLOGY; LABOR;
D O I
10.1016/j.jddst.2023.104409
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Developing an oral palatable emulsion of omega-3 fatty acids (omega-3) fortified with vitamins and minerals is a daunting task. Presently, such a combination is not available in the market. Therefore, the present study focuses to develop o/w emulsion for omega-3 fortified with essential vitamins and minerals with better physio-chemical stability, organoleptic properties, and bioaccessibility. The optimized formulation was milky yellowish having 146 +/- 28 nm globule size, 0.372 polydispersity index,-17.2 +/- 3.8 mV zeta potential, 3.74 +/- 0.1 pH, 990 +/- 20 cP viscosity with improved oxidative stability and bioaccessibility. Peroxide content and rancimat analysis displayed an increase in oxidative stability whereas, in-vitro DHA absorption and in-vivo serum FTIC-dextran permeability studies confirmed the better oral bioaccessibility for optimized-formulation in comparison to pure oil and commercially available counterpart. Further, in-silico computational screening revealed a high af-finity of DHA with AKR1C3 enzyme that may lead to induction of uterine contraction thus helps in preventing preterm births (PTB). In addition, results from in-vivo pharmacodynamic studies demonstrated a 60.02% reduction in PTB cases when treated with the fortified omega-3 formulation in comparison to the lipopolysaccharide treated positive control group. Thus, the results demonstrated a novel fortified oral palatable emulsion of omega-3 that can maintain a healthy pregnancy and manage the risk of PTB.
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页数:14
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