Phenology and ecological role of aerobic anoxygenic phototrophs in freshwaters

被引:5
|
作者
Villena-Alemany, Cristian [1 ,2 ]
Mujakic, Izabela [1 ]
Fecskeova, Livia K. [3 ]
Woodhouse, Jason [4 ]
Auladell, Adria [5 ]
Dean, Jason [1 ]
Hanusova, Martina [1 ]
Socha, Magdalena [6 ]
Gazulla, Carlota R. [7 ]
Ruscheweyh, Hans-Joachim [8 ,9 ]
Sunagawa, Shinichi [8 ,9 ]
Kavagutti, Vinicius Silva [2 ,9 ,10 ]
Andrei, Adrian-Stefan [11 ]
Grossart, Hans-Peter [4 ,12 ]
Ghai, Rohit [10 ]
Koblizek, Michal [1 ,2 ]
Piwosz, Kasia [6 ]
机构
[1] Czech Acad Sci, Lab Anoxygen Phototrophs, Inst Microbiol, Trebon, Czech Republic
[2] Univ South Bohemia, Fac Sci, Dept Ecosyst Biol, Ceske Budejovice, Czech Republic
[3] Pavol Jozef Safarik Univ Kosice, Fac Med, Associated Tissue Bank, Kosice, Slovakia
[4] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Plankton & Microbial Ecol, Stechlin, Germany
[5] Inst Evolutionary Biol, Funct Genom & Evolut Dept, CSIC UPF, Barcelona, Catalonia, Spain
[6] Natl Marine Fisheries Res Inst, Dept Fisheries Oceanog & Marine Ecol, Gdynia, Poland
[7] IEO CSIC, Inst Espanol Oceanog, Fuengirola 29640, Malaga, Spain
[8] Inst Microbiol, Swiss Inst Bioinformat, ETH Zurich, Zurich, Switzerland
[9] Swiss Fed Inst Technol, Swiss Inst Bioinformat, Zurich, Switzerland
[10] Biol Ctr CAS, Inst Hydrobiol, Dept Aquat Microbial Ecol, Ceske Budejovice, Czech Republic
[11] Univ Zurich, Dept Plant & Microbial Biol, Limnol Stn, Kilchberg, Switzerland
[12] Potsdam Univ, Inst Biol & Biochem, Potsdam, Germany
关键词
Freshwaters; Aquatic microbial ecology; Microbial seasonal succession; Long-term sampling; Aerobic anoxygenic phototrophs; pufM gene; PEG model; Photoheterotrophs; SEMI-MICRO DETERMINATION; PEG-MODEL; DIVERSITY; BACTERIA; GENE; GROWTH; POPULATIONS; EVOLUTION; ATLANTIC; DATABASE;
D O I
10.1186/s40168-024-01786-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background Aerobic anoxygenic phototrophic (AAP) bacteria are heterotrophic bacteria that supply their metabolism with light energy harvested by bacteriochlorophyll-a-containing reaction centers. Despite their substantial contribution to bacterial biomass, microbial food webs, and carbon cycle, their phenology in freshwater lakes remains unknown. Hence, we investigated seasonal variations of AAP abundance and community composition biweekly across 3 years in a temperate, meso-oligotrophic freshwater lake.Results AAP bacteria displayed a clear seasonal trend with a spring maximum following the bloom of phytoplankton and a secondary maximum in autumn. As the AAP bacteria represent a highly diverse assemblage of species, we followed their seasonal succession using the amplicon sequencing of the pufM marker gene. To enhance the accuracy of the taxonomic assignment, we developed new pufM primers that generate longer amplicons and compiled the currently largest database of pufM genes, comprising 3633 reference sequences spanning all phyla known to contain AAP species. With this novel resource, we demonstrated that the majority of the species appeared during specific phases of the seasonal cycle, with less than 2% of AAP species detected during the whole year. AAP community presented an indigenous freshwater nature characterized by high resilience and heterogenic adaptations to varying conditions of the freshwater environment.Conclusions Our findings highlight the substantial contribution of AAP bacteria to the carbon flow and ecological dynamics of lakes and unveil a recurrent and dynamic seasonal succession of the AAP community. By integrating this information with the indicator of primary production (Chlorophyll-a) and existing ecological models, we show that AAP bacteria play a pivotal role in the recycling of dissolved organic matter released during spring phytoplankton bloom. We suggest a potential role of AAP bacteria within the context of the PEG model and their consideration in further ecological models.
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页数:14
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