Enzymatic synthesis of β-D-fructofuranosyl α-D-glucopyranosyl-(1→2)-α-D-glucopyranoside using Escherichia coli glycoside phosphorylase YcjT

被引:2
|
作者
Isono, Naoto [1 ]
Yagura, Shuichiro [2 ]
Yamanaka, Kanon [2 ]
Masuda, Yukino [2 ]
Mukai, Kazuki [2 ]
Katsuzaki, Hirotaka [1 ]
机构
[1] Mie Univ, Grad Sch Bioresources, Tsu, Mie, Japan
[2] Mie Univ, Fac Bioresources, Tsu, Mie, Japan
关键词
glycoside phosphorylase; kojibiose phosphorylase; maltose phosphorylase; non-reducing sugar; prebiotic; KOJIBIOSE; OLIGOSACCHARIDES; GLUCOSYLSUCROSE;
D O I
10.1093/bbb/zbad099
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using beta-D-glucose 1-phosphate as a donor. The product was identified as beta-D-fructofuranosyl alpha-D-glucopyranosyl-(1 -> 2)-alpha-D-glucopyranoside. This sugar was also synthesized from sucrose and maltose using YcjT and maltose phosphorylase and promoted the growth of the probiotic Bifidobacterium breve
引用
收藏
页码:1249 / 1253
页数:5
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