Label-free fluorescence detection of alkaline phosphatase activity using a G-triplex based dumbbell-shaped probe

被引:3
|
作者
Zhu, Wenping [1 ]
Li, Zhiyi [1 ]
Dai, Liyan [1 ]
Yang, Weijie [1 ]
Li, Yanxia [1 ]
机构
[1] Zhoukou Normal Univ, Coll Chem & Chem Engn, Zhoukou 466001, Peoples R China
关键词
G-triplex; Alkaline phosphatase; Dumbbell-shaped probe; Thioflavin T; DNA; STRATEGY; ASSAY;
D O I
10.1007/s44211-022-00241-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Based on the fluorescence enhancement property of the G-triplex (G3)-Thioflavin T (ThT) complex, a fluorescent biosensor was successfully constructed for detection of ALP using a G3-based dumbbell-shaped probe (DP). In this work, calf intestinal ALP (CIP) can act on the 5'-terminal phosphate of DP, thereby regulating the subsequent DNA ligation reaction and enzyme cleavage of the DP nick. When the DP is digested by exonuclease, the released G3 can bind to ThT, resulting in enhanced fluorescence signal. The linear range of the sensor for CIP detection is 0.00002-0.002 U/mu L, and the detection limit is 1.8 x 10(-5) U/mu L. The proposed method has the advantages of simplicity, no fluorophore labeling, and low cost, which was successfully applied to the screening of enzyme inhibitors and ALP determination in human serum samples. To the best of our knowledge, this is the first report of a biosensor using G3-ThT as the signal indicator for ALP detection, which should promote the further exploitation of applying G3-ThT complex in the field of various targets recognition and analysis.
引用
收藏
页码:297 / 302
页数:6
相关论文
共 50 条
  • [41] Nucleic acid-controlled quantum dots aggregation: A label-free fluorescence turn-on strategy for alkaline phosphatase detection
    Hu, Zhenzhen
    Chen, Jian
    Li, Yongxin
    Wang, Yan
    Zhang, Qingfeng
    Hussain, Ejaz
    Yang, Meiding
    Shahzad, Sohail Anjum
    Yu, Donghong
    Yu, Cong
    TALANTA, 2017, 169 : 64 - 69
  • [42] Highly Sensitive and Label-free Fluorescence Detection of DNA Based on Structure Switch of Hairpin Probe DNA to G-Quadruplex-based DNAzyme
    Song Lu-Na
    Zhang Yong-Hua
    Bo Hong-Yan
    Gao Qiang
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2015, 43 (09) : 1402 - 1407
  • [43] One-pot, one-step, label-free miRNA detection method based on the structural transition of dumbbell probe
    Jeung, Jae Hoon
    Han, Hyogu
    Jang, Se Hee
    Lee, Chang Yeol
    Ahn, Jun Ki
    TALANTA, 2024, 274
  • [44] Label-free detection of exonuclease III activity and its inhibition based on DNA hairpin probe
    Jiang, Xiafei
    Liu, Haisheng
    Khusbu, Farjana Yeasmin
    Ma, Changbei
    Ping, Anqi
    Zhang, Qiling
    Wu, Kefeng
    Chen, Mingjian
    ANALYTICAL BIOCHEMISTRY, 2018, 555 : 55 - 58
  • [45] Analysis of the dephosphorylation kinetics of β-casein by alkaline phosphatase using a label-free quantitation method
    Oses-Prieto, J.
    Guan, S.
    Maltby, D. A.
    Burlingame, A. L.
    MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (08) : 29 - 29
  • [46] Ratiometric detection and imaging of endogenous alkaline phosphatase activity by fluorescein-coumarin-based fluorescence probe
    Cao, Jingrui
    Wu, Qiong
    Chang, Xin
    Chu, Hongyu
    Zhang, Hong
    Fang, Xuedong
    Chen, Fangfang
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 281
  • [47] Ratiometric detection and imaging of endogenous alkaline phosphatase activity by fluorescein-coumarin-based fluorescence probe
    Cao, Jingrui
    Wu, Qiong
    Chang, Xin
    Chu, Hongyu
    Zhang, Hong
    Fang, Xuedong
    Chen, Fangfang
    Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2022, 281
  • [48] A sensitive SERS detection of miRNA using a label-free multifunctional probe
    Zhang, Hao
    Liu, Yu
    Gao, Jian
    Zhen, Junhui
    CHEMICAL COMMUNICATIONS, 2015, 51 (94) : 16836 - 16839
  • [49] Label-free aptasensor for ochratoxin A detection using SYBR Gold as a probe
    Lv, Lei
    Li, Donghao
    Liu, Renjie
    Cui, Chengbi
    Guo, Zhijun
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 246 : 647 - 652
  • [50] A Label-Free Microfluidic Biosensor for Activity Detection of Single Microalgae Cells Based on Chlorophyll Fluorescence
    Wang, Junsheng
    Sun, Jinyang
    Song, Yongxin
    Xu, Yongyi
    Pan, Xinxiang
    Sun, Yeqing
    Li, Dongqing
    SENSORS, 2013, 13 (12) : 16075 - 16089