This study aims to provide information about the chemical profile of the essential oil from C. langsdorffii resin in areas of Cerradao, Carrasco, and Humid Forest. In order to obtain the essential oils, oil-resin was submitted to hydrodistillation process, and the chemical components were analyzed by gas chromatography coupled to mass spectrometry (GC/MS). Twenty-six constituents were identified in the essential oil of C. langsdorffii resin, of which 12 were present in Cerradao, 15 in Carrasco and 18 in Humid Forest, with beta-bisabolene, caryophyllene oxide, gamma-muurolene, alpha-caryophyllene and beta-caryophyllene common to the three phytophysiognomies. Among the studied areas, the resin collection varied from 2.652g to 20.263g, while the essential oil yield varied from 2.216 % to 11.764 %. A concentration range of 0.60 % to 84.57 % was recorded among the compounds in the three study areas. There was variation in chemical composition both among phytophysiognomies and among individuals from the same location, where beta-caryophyllene showed to be the majority for all areas studied, being present in all individuals from Cerradao, Carrasco and Humid Forest.