Protocol for lentivirus-mediated delivery of genes to study neurogenesis and cognitive function in adult rodents

被引：3

作者：

Xu, Li ^{[1
]}

Tang, Changyong ^{[1
]}

机构：

[1] SUN Yat Sen Univ, Affiliated Hosp 3, Dept Neurol, 600 Tianhe Rd, Guangzhou 510630, Guangdong, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

关键词：

Behavior; Cognitive Neuroscience;

D O I：

10.1016/j.xpro.2023.102761

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adult neurogenesis leads to the generation of functional neurons from neural stem cells, whereas impairment of adult hippocampal neurogenesis contributes to the pathophysiology of cognitive symptoms in neurodegenerative and neuropsychiatric diseases. Here, we present a protocol for a direct hippocampal injection of lentivirus-delivered gene in adult rodents to study the specific molecular mechanism underlying adult neurogenesis, including lentivirus packaging and stereotaxic injection, EdU and BrdU injections, tissue immunostaining and imaging analysis, and cognitive testing. For complete details on the use and execution of this protocol, please refer to Li et al. (2023).1

引用

页数：19

共 50 条

[21] Lentivirus-mediated transgene delivery to the hippocampus reveals sub-field specific differences in expression
Lenneke WA van Hooijdonk
Muhammad Ichwan
Thomas F Dijkmans
Theo G Schouten
Marijke WA de Backer
Roger AH Adan
Fons J Verbeek
Erno Vreugdenhil
Carlos P Fitzsimons
BMC Neuroscience, 10
[22] Lentivirus-mediated Knockdown of Ski Improves Neurological Function After Spinal Cord Injury in Rats
Wang, Zhi-Qiang
Ran, Rui
Ma, Chun-Wei
Zhao, Guang-Hai
Zhou, Kai-Sheng
Zhang, Hai-Hong
NEUROCHEMICAL RESEARCH, 2025, 50 (01)
[23] Activation of Transgene-specific T Cells Following Lentivirus-mediated Gene Delivery to Mouse Lung
Limberis, Maria P.
Bell, Christie L.
Heath, Jack
Wilson, James M.
MOLECULAR THERAPY, 2010, 18 (01) : 143 - 150
[24] REGULATION AND FUNCTION OF ADULT NEUROGENESIS: FROM GENES TO COGNITION
Aimone, James B.
Li, Yan
Lee, Star W.
Clemenson, Gregory D.
Deng, Wei
Gage, Fred H.
PHYSIOLOGICAL REVIEWS, 2014, 94 (04) : 991 - 1026
[25] Nutritional Factors Affecting Adult Neurogenesis and Cognitive Function
Poulose, Shibu M.
Miller, Marshall G.
Scott, Tammy
Shukitt-Hale, Barbara
ADVANCES IN NUTRITION, 2017, 8 (06) : 804 - 811
[26] Silencing of Choline Acetyltransferase Expression by Lentivirus-Mediated RNA Interference in Cultured Cells and in the Adult Rodent Brain
Santamaria, Julie
Khalfallah, Olfa
Sauty, Claire
Brunet, Isabelle
Sibieude, Mathieu
Mallet, Jacques
Berrard, Sylvie
Lecomte, Marie-Jose
JOURNAL OF NEUROSCIENCE RESEARCH, 2009, 87 (02) : 532 - 544
[27] Effect of tryptase inhibition on joint inflammation: a pharmacological and lentivirus-mediated gene transfer study
Alexandre Denadai-Souza
Camilla Moreira Ribeiro
Corinne Rolland
Anne Thouard
Céline Deraison
Cristoforo Scavone
Daniel Gonzalez-Dunia
Nathalie Vergnolle
Maria Christina Werneck Avellar
Arthritis Research & Therapy, 19
[28] Effect of tryptase inhibition on joint inflammation: a pharmacological and lentivirus-mediated gene transfer study
Denadai-Souza, Alexandre
Ribeiro, Camilla Moreira
Rolland, Corinne
Thouard, Anne
Deraison, Celine
Scavone, Cristoforo
Gonzalez-Dunia, Daniel
Vergnolle, Nathalie
Werneck Avellar, Maria Christina
ARTHRITIS RESEARCH & THERAPY, 2017, 19
[29] Lentivirus-mediated SERCA2a gene transfer improves left ventricular function in heart failure
Mattila, M.
Koskenvuo, J.
Savontaus, M.
HUMAN GENE THERAPY, 2015, 26 (10) : A40 - A41
[30] Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery
Jacobsen, M.
Askou, A. L.
Stenderup, K.
Rosada, C.
Dagnaes-Hansen, F.
Jensen, T. G.
Corydon, T. J.
Mikkelsen, J. G.
Aagaard, L.
HUMAN GENE THERAPY, 2015, 26 (10) : A102 - A102

← 1 2 3 4 5 →