Microorganisms on the surface are important contributors to producing a serious threat to global public health. Herein, we introduce an electro-thermal antimicrobial carbon surface. When a 9 V direct voltage was applied to the carbon surface, the carbon fibre surface temperature reached 80 degrees C. This electrothermal-based antimicrobial carbon surface showed outstanding inactivation performance, exhibiting a potent inactivation rate of similar to 99.99% against Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli) in just 10 min. Interestingly, under the same temperature, when alternating voltages were applied, electro-thermal antimicrobial performance was enhanced, resulting in a > 6.0 log reduction against both Grampositive and Gram-negative bacteria. In addition, under alternating voltage applied conditions, this excellent electro-thermal-based antimicrobial performance was maintained even at a lower surface temperature (50 degrees C).