Predicting Collagen Triple Helix Stability through Additive Effects of Terminal Residues and Caps

被引：11
|
作者：
Fiala, Tomas [1 ]
Barros, Emilia P. [2 ]
Heeb, Rahel [1 ]
Riniker, Sereina [2 ]
Wennemers, Helma [1 ]
机构：
[1] Swiss Fed Inst Technol, Lab Organ Chem, Vladimir Prelog Weg 3, CH-8093 Zurich, Switzerland
[2] Swiss Fed Inst Technol, Lab Phys Chem, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2023年 / 62卷 / 03期
基金：
瑞士国家科学基金会;
关键词：
Collagen; n?pi* Interaction; Peptides; Terminal Capping Group; Thermal Stability; CONFORMATIONAL STABILITY; CRYSTAL-STRUCTURE; MODEL PEPTIDES; CYSTINE KNOTS; TRIMERS; PROLINE;
D O I：
10.1002/anie.202214728
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Collagen model peptides (CMPs) consisting of proline-(2S,4R)-hydroxyproline-glycine (POG) repeats have provided a breadth of knowledge of the triple helical structure of collagen, the most abundant protein in mammals. Predictive tools for triple helix stability have, however, lagged behind since the effect of CMPs with different frames ([POG](n), [OGP](n), or [GPO](n)) and capped or uncapped termini have so far been underestimated. Here, we elucidated the impact of the frame, terminal functional group and its charge on the stability of collagen triple helices. Combined experimental and theoretical studies with frame-shifted, capped and uncapped CMPs revealed that electrostatic interactions, strand preorganization, interstrand H-bonding, and steric repulsion at the termini contribute to triple helix stability. We show that these individual contributions are additive and allow for the prediction of the melting temperatures of CMP trimers.
引用
收藏
页数：10
相关论文
共 50 条
  • [31] Collagen model peptides: Sequence dependence of triple-helix stability
    Persikov, Anton V.
    Ramshaw, John A. M.
    Brodsky, Barbara
    Biopolymers - Peptide Science Section, 2000, 55 (06): : 436 - 450
  • [32] Tuning of Collagen Triple-Helix Stability in Recombinant Telechelic Polymers
    Silva, Catarina I. F.
    Skrzeszewska, Paulina J.
    Golinska, Monika D.
    Werten, Marc W. T.
    Eggink, Gerrit
    de Wolf, Frits A.
    BIOMACROMOLECULES, 2012, 13 (05) : 1250 - 1258
  • [33] Effect of Sterically Demanding Substituents on the Conformational Stability of the Collagen Triple Helix
    Erdmann, Roman S.
    Wennemers, Helma
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (41) : 17117 - 17124
  • [34] The Thermal Stability of the Collagen Triple Helix Is Tuned According to the Environmental Temperature
    Fujii, Kazunori K.
    Taga, Yuki
    Takagi, Yusuke K.
    Masuda, Ryo
    Hattori, Shunji
    Koide, Takaki
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (04)
  • [35] Contribution of dipole-dipole interactions to the stability of the collagen triple helix
    Improta, Roberto
    Berisio, Rita
    Vitagliano, Luigi
    PROTEIN SCIENCE, 2008, 17 (05) : 955 - 961
  • [36] Collagen model peptides: Sequence dependence of triple-helix stability
    Persikov, AV
    Ramshaw, JAM
    Brodsky, B
    BIOPOLYMERS, 2000, 55 (06) : 436 - 450
  • [37] Decoding Collagen Triple Helix Stability by Means of Hybrid DFT Simulations
    Cutini, Michele
    Bocus, Massimo
    Ugliengo, Piero
    JOURNAL OF PHYSICAL CHEMISTRY B, 2019, 123 (34): : 7354 - 7364
  • [38] THE EFFECT OF A GLY -] ALA MUTATION ON COLLAGEN TRIPLE-HELIX STABILITY
    WEINSTEIN, JA
    PATERLINI, G
    NEMETHY, G
    SCHERAGA, HA
    BIOPHYSICAL JOURNAL, 1994, 66 (02) : A61 - A61
  • [39] The effect of fluoroproline in the X-position on the stability of the collagen triple helix
    Hodges, JA
    Raines, RT
    BIOPOLYMERS, 2003, 71 (03) : 312 - 312
  • [40] DIFFERENT EFFECTS OF 4-HYDROXYPROLINE AND 4-FLUOROPROLINE ON THE THERMAL STABILITY OF COLLAGEN TRIPLE HELIX
    Nishi, Y.
    Uchiyama, S.
    Doi, M.
    Nishiuchi, Y.
    Nakazawa, T.
    Ohkubo, T.
    Kobayashi, Y.
    JOURNAL OF PEPTIDE SCIENCE, 2004, 10 : 277 - 277
12345