The METTL3/miR-196a Axis Predicts Poor Prognosis in Non-small Cell Lung Cancer

被引:1
|
作者
Yang, Zhen [1 ,2 ]
Hao, Jie [4 ]
Qiu, Minghan [2 ]
Liu, Ruxue [2 ,3 ]
Mei, Hanwei [2 ,3 ]
Zhang, Qiaonan [4 ]
Gao, Zhanhua [4 ]
Pang, Wenwen
Liu, Jing [1 ]
Pan, Wenjie [1 ]
Wang, Huaqing [2 ,3 ]
Gao, Ming [4 ]
机构
[1] Nankai Univ, Tianjin Union Med Ctr, Dept Clin Lab, Tianjin 300121, Peoples R China
[2] Nankai Univ, Inst Translat Med, Tianjin Union Med Ctr, Tianjin 300121, Peoples R China
[3] Nankai Univ, Tianjin Union Med Ctr, Dept Oncol, Tianjin 300121, Peoples R China
[4] Nankai Univ, Tianjin Union Med Ctr, Dept Thyroid & Breast Surg, Tianjin Key Lab Gen Surg Construct, Tianjin 300121, Peoples R China
来源
JOURNAL OF CANCER | 2024年 / 15卷 / 06期
基金
中国国家自然科学基金;
关键词
METTL3; m6A; miR-196a; GAS7; NSCLC; immune cell infiltration; GAS7;
D O I
10.7150/jca.92968
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: METTL3 accelerates m6A modification to influence cancer progression including non-small cell lung cancer (NSCLC). To illustrate the role and underlying mechanism of METTL3 mediated miR-196a upregulation in NSCLC. Method: The global level of m6A modification was detected by qPCR, western blot and immumohistochemical staining. The TCGA, GEPIA, CPTAC and TIMER databases were used to explore the expression change of METTL3, miR-196a and GAS7 in NSCLC patients. Kaplan-Meier analysis was performed to analyze the prognostic value of miR-196a. NSCLC cells overexpressed or knockdown miR-196a were constructed and used for CCK8, colony formation assay, western blot and immunofluorescence in vitro. The effect of miR-196a on tumor growth was investigated in vivo. Result: We found that METTL3 mediated miR-196a were notably enhancive in NSCLC tissues and in NSCLC cells, which is markedly positively related with the serious TNM stage, the large tumor size, the distant metastasis, and the poor prognosis in patients of NSCLC. Further investigation showed that up-regulated miR-196a promoted cell viability and cell autophagy, while down-regulation of miR-196a revealed opposite results in H1299 and A549 cells. In terms of mechanism, we found that miR-196a interacted with GAS7. In addition, GAS7 expression in NSCLC patients may be positively related with the infiltration of immune cell subsets in tumor microenvironment (TME). Conclusion: The axis of METTL3-miR-196a-GAS7 might be a target for molecular targeted therapy, a potential and novel diagnostic marker for NSCLC patients.
引用
收藏
页码:1603 / 1612
页数:10
相关论文
共 50 条
  • [21] SMOX expression predicts the prognosis of non-small cell lung cancer
    Huang, Zhanghao
    Wang, Shuo
    Zhang, Hai-Jian
    Zhou, You Lang
    Shi, Jia-Hai
    ANNALS OF TRANSLATIONAL MEDICINE, 2021, 9 (13)
  • [22] Decreased SARI expression predicts poor prognosis of Chinese patients with non-small cell lung cancer
    Zhou, Ren-Jie
    Shi, Zhong
    Zhou, Kun
    Wang, Hai-Dong
    Zhang, Guo-Qiang
    Li, Xue-Tao
    Xu, Jian-Ping
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2013, 6 (10): : 2056 - 2063
  • [23] High Sam68 expression predicts poor prognosis in non-small cell lung cancer
    Z. Zhang
    Y. Xu
    N. Sun
    M. Zhang
    J. Xie
    Z. Jiang
    Clinical and Translational Oncology, 2014, 16 : 886 - 891
  • [24] LncRNA GHET1 predicts a poor prognosis of the patients with non-small cell lung cancer
    Shen, Q-M
    Wang, H-Y
    Xu, S.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2018, 22 (08) : 2328 - 2333
  • [25] PPM1D overexpression predicts poor prognosis in non-small cell lung cancer
    Yang, Hua
    Gao, Xiao-Yu
    Li, Ping
    Jiang, Ting-Shu
    TUMOR BIOLOGY, 2015, 36 (03) : 2179 - 2184
  • [26] High Sam68 expression predicts poor prognosis in non-small cell lung cancer
    Zhang, Z.
    Xu, Y.
    Sun, N.
    Zhang, M.
    Xie, J.
    Jiang, Z.
    CLINICAL & TRANSLATIONAL ONCOLOGY, 2014, 16 (10): : 886 - 891
  • [27] A Low Advanced Lung Cancer Inflammation Index Predicts a Poor Prognosis in Patients With Metastatic Non-Small Cell Lung Cancer
    Lu, Ping
    Ma, Yifei
    Kai, Jindan
    Wang, Jun
    Yin, Zhucheng
    Xu, Hongli
    Li, Xinying
    Liang, Xin
    Wei, Shaozhong
    Liang, Xinjun
    FRONTIERS IN MOLECULAR BIOSCIENCES, 2022, 8
  • [28] High Expression of lncRNA HEIH is Helpful in the Diagnosis of Non-Small Cell Lung Cancer and Predicts Poor Prognosis
    He, Chaowen
    Huang, Dongxuan
    Yang, Fan
    Huang, Dongsheng
    Cao, Yahui
    Peng, Jianfeng
    Luo, Xiaohua
    CANCER MANAGEMENT AND RESEARCH, 2022, 14 : 503 - 514
  • [29] Overexpression of CDC20 predicts poor prognosis in primary non-small cell lung cancer patients
    Kato, Tatsuya
    Daigo, Yataro
    Aragaki, Masato
    Ishikawa, Keidai
    Sato, Masaaki
    Kaji, Mitsuhito
    JOURNAL OF SURGICAL ONCOLOGY, 2012, 106 (04) : 423 - 430
  • [30] Enhanced expression of FGF19 predicts poor prognosis in patients with non-small cell lung cancer
    Chen, Jia
    Shao, Jingjing
    Shen, Aiguo
    Zhu, Xinghua
    Zhang, Xunlei
    Sun, Hui
    Wei, Sheng
    Ling, Yang
    JOURNAL OF THORACIC DISEASE, 2021, 13 (03) : 1769 - 1784