Quantitative cytofluorimetric analysis of mouse neutrophil extracellular traps

被引：0
|
作者：
Poli, Valentina [1 ,2 ]
Gioia, Marco Di [1 ,2 ]
Zanoni, Ivan [1 ,2 ,3 ]
机构：
[1] Harvard Med Sch, Boston, MA 02115 USA
[2] Boston Childrens Hosp, Div Immunol, Boston, MA 02115 USA
[3] Boston Childrens Hosp, Div Gastroenterol, Boston, MA 02115 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 03期
关键词：
Immunology; Microbiology;
D O I：
10.1016/j.xpro.2023.102372
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The release of neutrophil extracellular traps (NETs) has been involved in numerous infectious and non-infectious diseases. Nevertheless, quantitative analysis of NETs in vivo has been challenging. Here, we present a protocol for NET quantification by flow cytometry in the bronchoalveolar lavage fluid (BALF) of mice upon pulmonary infection with S. aureus. We describe steps for bacteria growth and instillation and BALF recovery. We then detail staining to quantify the release of NETs and neutrophils recruited to the site of infection. For complete information on the generation and use of this protocol, please refer to Poli et al. (2021)(1) and Poli et al. (2022).(2)
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页数：10
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