Protocol for the isolation of GFP-expressing ferroptosis-dependent extracellular vesicles in in vitro cell culture models

被引：0

作者：

Ito, Fumiya ^{[1
,4
]}

Yanatori, Izumi ^{[2
]}

Kato, Katsuhiro ^{[3
]}

Toyokuni, Shinya ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Pathol & Biol Responses, 65 Tsurumai Cho,Showa Ku, Nagoya 4668550, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Mol & Cellular Physiol, Kyoto, Japan

[3] Nagoya Univ, Grad Sch Med, Dept Cardiol, 65 Tsurumai Cho,Showa Ku, Nagoya 4668550, Japan

[4] Johns Hopkins Univ, Sch Med, Dept Cell Biol, Baltimore, MD 21205 USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 01期

关键词：

IRON;

D O I：

10.1016/j.xpro.2024.102892

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular vesicles (EVs) are complex structures that transport various DNA, RNA, and protein. Recently, new EV secretion mechanisms have been identified through the iron regulatory system in mammalian cells. We revealed that ferroptosis increases EV secretion, which is named ferroptosis-dependent EVs (FedEVs). Here, we describe a step-by-step procedure to isolate GFP-expressing FedEVs for in vitro analysis. The FedEVs are further analyzed by imaging and flow cytometry analysis. For complete details on the use and execution of this protocol, please refer to Ito et al.1

引用

页数：18

共 32 条

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