Interferon-λ3 alleviates intestinal epithelium injury induced by porcine rotavirus in mice

被引:0
|
作者
Wang, Yuhan
Yu, Bing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Huang, Zhiqing
Yu, Jie
Luo, Junqiu
Yan, Hui
Wu, Aimin
He, Jun [1 ]
机构
[1] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
IFN-lambda; 3; Innate immunity; Intestinal epithelium; Infection; Regulation; IFN-LAMBDA; ANTIVIRAL ACTIVITY; STIMULATED GENES; MUCOSAL IMMUNITY; TIGHT JUNCTIONS; INFECTION; PROTECTION; MODEL; PERMEABILITY; PATHOGENESIS;
D O I
10.1016/j.ijbiomac.2023.124431
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interferons are a group of glycoproteins that are expressed in various cell types in their inflammatory responses to infections. In this study, we explored the protective effects of porcine interferon-lambda 3 (PIFN-lambda 3) on intestinal inflammation and injury in mice induced by porcine rotavirus (PRV). BALB/c mice were administrated by PIFN-lambda 3 or phosphate buffer solution (PBS) for three days prior to PRV infection. We show that PRV infection caused acute inflammatory responses in mice, as indicated by increases in serum concentrations of inflammatory cytokines such as the interlukin-1 beta (IL-1 beta), interlukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) (P < 0.05). However, PIFN-lambda 3 administration not only decreased their concentrations but also elevated the concentrations of immunoglobulin (Ig) M and IgG in the PRV challenged mice (P < 0.05). PRV infection significantly decreased the jejunal villus height and the ratio of villus height to crypt depth (V/C); however, PIFN-lambda 3 treatment significantly elevated the villus height and the abundance of tight junction protein ZO-1 in the jejunum (P < 0.05). Moreover, PIFN-lambda 3 decreased the replication of PRV in the jejunal epithelium, but significantly increased the abundance of sIgA and the activities of maltase and sucrase in the PRV-challenged mice (P < 0.05). Interestingly, PIFN-lambda 3 elevated the expression levels of sodium/glucose cotransporter 1 (SGLT1) and mucin 2 (MUC2) in the PRVchallenged mice (P < 0.05). Moreover, PIFN-lambda 3 significantly increased the expression levels of IL-10, signal transducer and activator of transcription 1 (STAT1), and critical interferon-stimulated genes such as the 2'-5' oligoadenylate synthetase-like 1 (OASL1), interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) and radical S-adenosyl methionine domain containing 2 (RSAD2) in the jejunum upon PRV infection (P < 0.05). The anti-virus and anti-inflammatory effect of PIFN-lambda 3 should make it an attractive candidate to prevent various pathogen-induced bowel diseases.
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页数:10
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