Development of RNA Polymerase Ⅲ-Driven Reverse Genetics System for the Rescue of a Plant Rhabdovirus

被引：0

作者：

Xiaoyan Zhang ^{[1
]}

Kai Sun ^{[2
]}

Yan Liang ^{[1
]}

Chenglu Zhao ^{[1
]}

Zhenghe Li ^{[1
,3
,4
]}

机构：

[1] State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University

[2] Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University

[3] Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University

[4] Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University

来源：

Virologica Sinica | 2021年 / 05期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

D O I：

暂无

中图分类号：

S432.41 [];

学科分类号：

090401 ;

摘要：

Dear Editor,De novo generation of negative-stranded RNA viruses(NSVs) requires efficient transcription of integral viral RNAs with precise termini from cloned plasmids. Studies during the past 25 years with animal NSVs have established the bacteriophage T7 RNA polymerase (Pol)-and endogenous Pol I-based transcription systems as the most efficient platforms for recovery of recombinant NSVs(Bridgen 2012). Unfortunately,

引用

页码：1252 / 1255

页数：4

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