Assessment of limiting dietary amino acids in broiler chickens offered reduced crude protein diets

As lowering crude protein(CP) in poultry diets continues to minimize amino acid excess, it is important to understand the limiting order of amino acids and the impact of their deficiencies. Therefore, a pair of experiments were conducted to observe the effects of individual amino acid deletions on growth performance, carcass traits, and nutrient utilization. Both experiments involved 3 control diets based on wheat and soybean meal, including a 210.0 g/kg CP industry control(IC), 186.7 g/kg CP positive control(PC) supplemented with feed-grade amino acids to match the IC amino acid profile, 186.7 g/kg CP negative control(NC) with reducing N corrected apparent metabolizable energy(AMEN) by 0.5 MJ/kg and removing feed-grade amino acids beyond L-Lys-HCl, DL-Met, and L-Thr from PC. Ten deletion diets where the following supplemented amino acids were individually removed from the PC: Val, Ile, Leu, Trp, Arg,His, Phe + Tyr, glycine equivalence(Glyequi), Pro, and Energy(0.5 MJ/kg reduction in AMENof the PC). All diets were formulated to contain similar concentrations of digestible Lys, total sulfur amino acid(TSAA)and Thr. Experimental diets were offered to broiler chickens from 15 to 22 d post-hatch in a cage study(Exp. 1) to gain digestibility and nutrient utilization data; whereas they were offered from 15 to 35 d post-hatch in a floor-pen study(Exp. 2) to gain performance and carcass yield data. The removal of supplemented Val, Arg, and Ile resulted in reduction on broiler performance(P < 0.05), and the removal of Val, Arg, Ile, and Glyequinegatively influenced carcass traits(P < 0.05). Results from both experiments indicate that Val and Arg are co-limiting in wheat-soybean meal diets, but that Ile and Glyequimay potentially limit breast and thigh development.